| Literature DB >> 23722769 |
Travis J Gould1, Emil B Kromann, Daniel Burke, Martin J Booth, Joerg Bewersdorf.
Abstract
Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 ± 2.3 nm.Entities:
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Year: 2013 PMID: 23722769 PMCID: PMC3749882 DOI: 10.1364/OL.38.001860
Source DB: PubMed Journal: Opt Lett ISSN: 0146-9592 Impact factor: 3.776