Literature DB >> 23706502

Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis.

Kittappa Vinothkumar1, Ashima Kushwaha Bhardwaj, Thandavarayan Ramamurthy, Swapan Kumar Niyogi.   

Abstract

A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology.
Copyright © 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Diagnostics; Epidemiology; Haem-utilising gene; Haemolysin gene; Toxin-regulatory gene

Mesh：

Substances：

Year:  2013        PMID： 23706502     DOI： 10.1016/j.diagmicrobio.2013.04.005

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  8 in total

1.  Molecular Detection and Distribution of Six Medically Important Vibrio spp. in Selected Freshwater and Brackish Water Resources in Eastern Cape Province, South Africa.

Authors:  Oluwatayo E Abioye; Ayodeji Charles Osunla; Anthony I Okoh
Journal:  Front Microbiol       Date:  2021-06-02       Impact factor: 5.640

2.  A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus.

Authors:  Ruichao Li; Jiachi Chiou; Edward Wai-Chi Chan; Sheng Chen
Journal:  Front Microbiol       Date:  2016-01-28       Impact factor: 5.640

3.  Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technology.

Authors:  Dejing Liu; Bo Hu; Dingfa Peng; Shan Lu; Shunxiang Gao; Zhengang Li; Lianghua Wang; Binghua Jiao
Journal:  RSC Adv       Date:  2020-04-22       Impact factor: 4.036

4.  Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics.

Authors:  Hyun-Joong Kim; Ji-Oh Ryu; Shin-Young Lee; Ei-Seul Kim; Hae-Yeong Kim
Journal:  BMC Microbiol       Date:  2015-10-26       Impact factor: 3.605

Review 5.  Vibrio fluvialis: an emerging human pathogen.

Authors:  Thandavarayan Ramamurthy; Goutam Chowdhury; Gururaja P Pazhani; Sumio Shinoda
Journal:  Front Microbiol       Date:  2014-03-07       Impact factor: 5.640

6.  Rapid multiplex detection of 10 foodborne pathogens with an up-converting phosphor technology-based 10-channel lateral flow assay.

Authors:  Yong Zhao; Haoran Wang; Pingping Zhang; Chongyun Sun; Xiaochen Wang; Xinrui Wang; Ruifu Yang; Chengbin Wang; Lei Zhou
Journal:  Sci Rep       Date:  2016-02-17       Impact factor: 4.379

7.  Simultaneous Identification of Ten Bacterial Pathogens Using the Multiplex Ligation Reaction Based on the Probe Melting Curve Analysis.

Authors:  Yixiang Jiang; Lianhua He; Pingfang Wu; Xiaolu Shi; Min Jiang; Yinghui Li; Yiman Lin; Yaqun Qiu; Fang Bai; Yiqun Liao; Qingge Li; RenLi Zhang; Qinghua Hu
Journal:  Sci Rep       Date:  2017-07-19       Impact factor: 4.379

8.  Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis.

Authors:  Liuyang Hu; Bing Han; Qin Tong; Hui Xiao; Donglin Cao
Journal:  Can J Infect Dis Med Microbiol       Date:  2020-02-26       Impact factor: 2.471
  8 in total

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