Literature DB >> 23702824

Nicked-sleeve interface for two-dimensional capillary electrophoresis.

Ryan J Flaherty1, Bonnie J Huge, Spencer M Bruce, Oluwatosin O Dada, Norman J Dovichi.   

Abstract

We report an improved interface for two-dimensional capillary electrophoresis. This interface is based on capillary tubing and a Plexiglas chip, both of which were milled using a micro-dicing saw. The interface was evaluated and compared to a traditional interface design for both pseudo one-dimensional and two-dimensional capillary electrophoresis. We observe less than 70% transfer efficiency for the traditional design and greater than 90% transfer efficiency with this new interface.

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Year:  2013        PMID： 23702824      PMCID： PMC3697014          DOI： 10.1039/c3an00284e

Source DB:  PubMed          Journal:  Analyst        ISSN： 0003-2654            Impact factor:   4.616


  18 in total

1.  Reproducible two-dimensional capillary electrophoresis analysis of Barrett's esophagus tissues.

Authors:  James R Kraly; Megan R Jones; David G Gomez; Jane A Dickerson; Melissa M Harwood; Michael Eggertson; Thomas G Paulson; Carissa A Sanchez; Robert Odze; Ziding Feng; Brian J Reid; Norman J Dovichi
Journal:  Anal Chem       Date:  2006-09-01       Impact factor: 6.986

2.  Instrumentation for medium-throughput two-dimensional capillary electrophoresis with laser-induced fluorescence detection.

Authors:  Cuiru Zhu; Xinya He; James R Kraly; Megan R Jones; Colin D Whitmore; David G Gomez; Michael Eggertson; Wes Quigley; Anna Boardman; Norman J Dovichi
Journal:  Anal Chem       Date:  2007-01-15       Impact factor: 6.986

3.  Miniaturized two-dimensional capillary electrophoresis on a microchip for analysis of the tryptic digest of proteins.

Authors:  Yongzheng Cong; Lihua Zhang; Dingyin Tao; Yu Liang; Weibing Zhang; Yukui Zhang
Journal:  J Sep Sci       Date:  2008-02       Impact factor: 3.645

4.  Fractionation of the human recombinant tissue plasminogen activator (rtPA) glycoforms by high-performance capillary zone electrophoresis and capillary isoelectric focusing.

Authors:  K W Yim
Journal:  J Chromatogr       Date:  1991-10-18

5.  Analysis of differential detergent fractions of an AtT-20 cellular homogenate using one- and two-dimensional capillary electrophoresis.

Authors:  Md Abul Fazal; Vanessa R Palmer; Norman J Dovichi
Journal:  J Chromatogr A       Date:  2006-06-16       Impact factor: 4.759

6.  Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis.

Authors:  J P Liu; O Shirota; D Wiesler; M Novotny
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

7.  Capillary sieving electrophoresis/micellar electrokinetic capillary chromatography for two-dimensional protein fingerprinting of single mammalian cells.

Authors:  Shen Hu; David A Michels; Md Abul Fazal; Chootima Ratisoontorn; Michael L Cunningham; Norman J Dovichi
Journal:  Anal Chem       Date:  2004-07-15       Impact factor: 6.986

8.  Automated enzyme-based diagonal capillary electrophoresis: application to phosphopeptide characterization.

Authors:  Roza Wojcik; Michael Vannatta; Norman J Dovichi
Journal:  Anal Chem       Date:  2010-02-15       Impact factor: 6.986

9.  Chip-capillary hybrid device for automated transfer of sample preseparated by capillary isoelectric focusing to parallel capillary gel electrophoresis for two-dimensional protein separation.

Authors:  Joann J Lu; Shili Wang; Guanbin Li; Wei Wang; Qiaosheng Pu; Shaorong Liu
Journal:  Anal Chem       Date:  2012-08-03       Impact factor: 6.986

10.  CSE-MECC two-dimensional capillary electrophoresis analysis of proteins in the mouse tumor cell (AtT-20) homogenate.

Authors:  Xingguo Chen; Md Abul Fazal; Norman J Dovichi
Journal:  Talanta       Date:  2007-03-30       Impact factor: 6.057
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  3 in total

1.  Coupling immobilized alkaline phosphatase-based automated diagonal capillary electrophoresis to tandem mass spectrometry for phosphopeptide analysis.

Authors:  Si Mou; Liangliang Sun; Roza Wojcik; Norman J Dovichi
Journal:  Talanta       Date:  2013-08-16       Impact factor: 6.057

2.  Accurate determination of peptide phosphorylation stoichiometry via automated diagonal capillary electrophoresis coupled with mass spectrometry: proof of principle.

Authors:  Si Mou; Liangliang Sun; Norman J Dovichi
Journal:  Anal Chem       Date:  2013-11-05       Impact factor: 6.986

3.  Automatic Dissolution Testing with High-Temporal Resolution for Both Immediate-Release and Fixed-Combination Drug Tablets.

Authors:  Zhongmei Chi; Irfan Azhar; Habib Khan; Li Yang; Yunxiang Feng
Journal:  Sci Rep       Date:  2019-11-19       Impact factor: 4.379
  3 in total

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