Literature DB >> 1706520

Ultrasensitive fluorometric detection of carbohydrates as derivatives in mixtures separated by capillary electrophoresis.

J P Liu1, O Shirota, D Wiesler, M Novotny.   

Abstract

Reducing monosaccharides and oligosaccharides, after reductive amination, were separated and detected as their 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) derivatives by capillary electrophoresis/laser-induced fluorescence. Under optimized conditions, the minimum detectable quantities for monosaccharide solutes were assessed at low attomole levels (0.5 amol for the CBQCA derivative of galactose). The system has shown considerable promise for high-sensitivity analysis of both neutral and amino sugars in glycoproteins. Complex oligosaccharides, isolated from bovine fetuin by hydrazinolysis, were also successfully "mapped."

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Year:  1991        PMID： 1706520      PMCID： PMC51219          DOI： 10.1073/pnas.88.6.2302

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Capillary zone electrophoresis of oligonucleotides. Factors affecting separation.

Authors:  V Dolnik; J Liu; J F Banks; M V Novotny; P Bocek
Journal:  J Chromatogr       Date:  1989-10-20

2.  Capillary electrophoresis.

Authors:  A G Ewing; R A Wallingford; T M Olefirowicz
Journal:  Anal Chem       Date:  1989-02-15       Impact factor: 6.986

Review 3.  High-performance capillary electrophoresis in the biological sciences.

Authors:  B L Karger; A S Cohen; A Guttman
Journal:  J Chromatogr       Date:  1989-08-11

Review 4.  Glycoproteins: what are the sugar chains for?

Authors:  J C Paulson
Journal:  Trends Biochem Sci       Date:  1989-07       Impact factor: 13.807

5.  Simultaneous determination of reducing monosaccharides by capillary zone electrophoresis as the borate complexes of N-2-pyridylglycamines.

Authors:  S Honda; S Iwase; A Makino; S Fujiwara
Journal:  Anal Biochem       Date:  1989-01       Impact factor: 3.365

6.  Analysis of high molecular weight samples on a double-focusing magnetic sector instrument by supercritical fluid chromatography/mass spectrometry.

Authors:  V N Reinhold; D M Sheeley; J Kuei; G R Her
Journal:  Anal Chem       Date:  1988-12-15       Impact factor: 6.986

7.  Analysis of tissue glycoprotein sugar chains by two-dimensional high-performance liquid chromatographic mapping.

Authors:  S Hase; K Ikenaka; K Mikoshiba; T Ikenaka
Journal:  J Chromatogr       Date:  1988-12-29

8.  Common conditions for high-performance liquid chromatographic microdetermination of aldoses, hexosamines, and sialic acids in glycoproteins.

Authors:  S Honda; S Suzuki
Journal:  Anal Biochem       Date:  1984-10       Impact factor: 3.365

9.  Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.

Authors:  A S Cohen; D R Najarian; A Paulus; A Guttman; J A Smith; B L Karger
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

10.  Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection.

Authors:  M R Hardy; R R Townsend; Y C Lee
Journal:  Anal Biochem       Date:  1988-04       Impact factor: 3.365
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  16 in total

Review 1.  High-sensitivity analytical approaches for the structural characterization of glycoproteins.

Authors:  William R Alley; Benjamin F Mann; Milos V Novotny
Journal:  Chem Rev       Date:  2013-03-27       Impact factor: 60.622

2.  Microchip electrophoresis at elevated temperatures and high separation field strengths.

Authors:  Indranil Mitra; Steven P Marczak; Stephen C Jacobson
Journal:  Electrophoresis       Date:  2013-11-20       Impact factor: 3.535

3.  N-glycan profiling by microchip electrophoresis to differentiate disease states related to esophageal adenocarcinoma.

Authors:  Indranil Mitra; Zexi Zhuang; Yuening Zhang; Chuan-Yih Yu; Zane T Hammoud; Haixu Tang; Yehia Mechref; Stephen C Jacobson
Journal:  Anal Chem       Date:  2012-04-03       Impact factor: 6.986

Review 4.  Analysis of glycans derived from glycoconjugates by capillary electrophoresis-mass spectrometry.

Authors:  Yehia Mechref
Journal:  Electrophoresis       Date:  2011-12       Impact factor: 3.535

5.  Electromigration behavior of polysaccharides in capillary electrophoresis under pulsed-field conditions.

Authors:  J Sudor; M Novotny
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-15       Impact factor: 11.205

6.  Microchip electrophoresis of N-glycans on serpentine separation channels with asymmetrically tapered turns.

Authors:  Zexi Zhuang; Indranil Mitra; Ahmed Hussein; Milos V Novotny; Yehia Mechref; Stephen C Jacobson
Journal:  Electrophoresis       Date:  2011-01       Impact factor: 3.535

7.  Simultaneous quantification of sialyloligosaccharides from human milk by capillary electrophoresis.

Authors:  Yuanwu Bao; Libin Zhu; David S Newburg
Journal:  Anal Biochem       Date:  2007-07-07       Impact factor: 3.365

8.  Nicked-sleeve interface for two-dimensional capillary electrophoresis.

Authors:  Ryan J Flaherty; Bonnie J Huge; Spencer M Bruce; Oluwatosin O Dada; Norman J Dovichi
Journal:  Analyst       Date:  2013-07-07       Impact factor: 4.616

9.  Structural Characterization of Serum N-Glycans by Methylamidation, Fluorescent Labeling, and Analysis by Microchip Electrophoresis.

Authors:  Indranil Mitra; Christa M Snyder; Xiaomei Zhou; Margit I Campos; William R Alley; Milos V Novotny; Stephen C Jacobson
Journal:  Anal Chem       Date:  2016-09-02       Impact factor: 6.986

10.  Comparative profiling of N-glycans isolated from serum samples of ovarian cancer patients and analyzed by microchip electrophoresis.

Authors:  Indranil Mitra; William R Alley; John A Goetz; Jacqueline A Vasseur; Milos V Novotny; Stephen C Jacobson
Journal:  J Proteome Res       Date:  2013-08-28       Impact factor: 4.466
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