| Literature DB >> 17637850 |
Xingguo Chen1, Md Abul Fazal, Norman J Dovichi.
Abstract
Two-dimensional capillary electrophoresis was used for the separation of proteins and biogenic amines from the mouse AtT-20 cell line. The first-dimension capillary contained a TRIS-CHES-SDS-dextran buffer to perform capillary sieving electrophoresis, which is based on molecular weight of proteins. The second-dimension capillary contained a TRIS-CHES-SDS buffer for micel1ar electrokinetic capillary chromatography. After a 61 seconds preliminary separation, fractions from the first-dimension capillary were successively transferred to the second-dimension capillary, where they further separated by MECC. The two-dimensional separation required 60 minutes.Entities:
Year: 2007 PMID： 17637850 PMCID： PMC1920328 DOI： 10.1016/j.talanta.2006.09.004
Source DB: PubMed Journal: Talanta ISSN： 0039-9140 Impact factor: 6.057