Literature DB >> 236548

Size fractionation of double-stranded DNA by precipitation with polyethylene glycol.

J T Lis, R Schleif.   

Abstract

We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG+.. Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 times 10-7 to 1 times 10-5 daltons. The effects on PEG concentration, sodium chloride concentration, DNA concentration, pH, divalent ions, precipitation time, and centrifugal force have been determined. These studies show PEG precipitation offers a size fractionation method for DNA which is convenient, of high capacity, and applicable over a wide range of conditions. However, resolution is not high and separation of two species approaches 100% only if they differ in molecular mass by at least a factor of two.

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Year:  1975        PMID： 236548      PMCID： PMC342844          DOI： 10.1093/nar/2.3.383

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Rapid sedimentation of Escherichia coli in the presence of polyethylene glycol at 1g.

Authors:  D L Eshenbaugh; D Sens; E James
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

2.  Control elements in the DNA of bacteriophage lambda.

Authors:  T Maniatis; M Ptashne; R Maurer
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

3.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

4.  Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.

Authors:  K J Danna; G H Sack; D Nathans
Journal:  J Mol Biol       Date:  1973-08-05       Impact factor: 5.469

5.  Concentration and purification of vesicular stomatitis virus by polyethylene glycol "precipitation".

Authors:  J McSharry; R Benzinger
Journal:  Virology       Date:  1970-03       Impact factor: 3.616

6.  Different cyclic AMP requirements for induction of the arabinose and lactose operons of Escherichia coli.

Authors:  J T Lis; R Schleif
Journal:  J Mol Biol       Date:  1973-09-05       Impact factor: 5.469

7.  Isolation of pure lac operon DNA.

Authors:  J Shapiro; L Machattie; L Eron; G Ihler; K Ippen; J Beckwith
Journal:  Nature       Date:  1969-11-22       Impact factor: 49.962

8.  Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.

Authors:  K R Yamamoto; B M Alberts; R Benzinger; L Lawhorne; G Treiber
Journal:  Virology       Date:  1970-03       Impact factor: 3.616
  8 in total
  49 in total

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Journal:  Mol Biotechnol       Date:  2002-07       Impact factor: 2.695

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Authors:  Rafael D Rosengarten; Maria A Moreno; Fadi G Lakkis; Leo W Buss; Stephen L Dellaporta
Journal:  Mol Biol Evol       Date:  2010-10-21       Impact factor: 16.240

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Authors:  A Maruyama; M Oda; T Higashihara
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

4.  96-well polycarbonate-based microfluidic titer plate for high-throughput purification of DNA and RNA.

Authors:  Małgorzata A Witek; Mateusz L Hupert; Daniel S-W Park; Kirby Fears; Michael C Murphy; Steven A Soper
Journal:  Anal Chem       Date:  2008-03-21       Impact factor: 6.986

5.  Cloning of the δ-aminolevulinic acid synthase structural gene in yeast.

Authors:  M Arrese; E Carvajal; S Robison; A Sambunaris; A Panek; J Mattoon
Journal:  Curr Genet       Date:  1983-06       Impact factor: 3.886

6.  Preparative fractionation of DNA restriction fragments by high pressure column chromatography on RPC-5.

Authors:  A Landy; C Foeller; R Reszelbach; B Dudock
Journal:  Nucleic Acids Res       Date:  1976-10       Impact factor: 16.971

7.  Development of a recombinant protein-based enzyme-linked immunosorbent assay and its applications in field surveillance of rodent mice for presence of immunoglobulin G against Orientia tsutsugamushi.

Authors:  Yeau-Ching Wang; Ting-Yu Jian; Lih-Jeng Tarn; Yao-Wen Hung; Hai-Yuan Chao; Da-Der Ji; Hwan-Wun Liu
Journal:  Clin Diagn Lab Immunol       Date:  2003-05

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Authors:  Sergei V Shekhovtsov; Alexei V Katokhin; Konstantin V Romanov; Vladimir V Besprozvannykh; Konstantin P Fedorov; Natalia I Yurlova; Elena A Serbina; Paiboon Sithithaworn; Nikolai A Kolchanov; Viatcheslav A Mordvinov
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9.  Increased throughput by parallelization of library preparation for massive sequencing.

Authors:  Sverker Lundin; Henrik Stranneheim; Erik Pettersson; Daniel Klevebring; Joakim Lundeberg
Journal:  PLoS One       Date:  2010-04-06       Impact factor: 3.240

10.  U3 snoRNA genes are multi-copy and frequently linked to U5 snRNA genes in Euglena gracilis.

Authors:  J Michael Charette; Michael W Gray
Journal:  BMC Genomics       Date:  2009-11-16       Impact factor: 3.969
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