Literature DB >> 23637494

Detection of Rotavirus Infection in Bovine Calves by RNA-PAGE and RT-PCR.

Sanjay Singh Basera1, Rashmi Singh, N Vaid, K Sharma, S Chakravarti, Y P S Malik.   

Abstract

A total of 128 diarrhoeic faecal samples were collected from cattle and buffalo calves from Pantnagar and Dehradun during winter months. Of the 110 cattle calves screened by RNA-PAGE, rotavirus was detected in 13 samples (11.81%) while no sample from buffalo calves was found positive. All samples were found to have long electropherotype and two distinct electropherotypes having segment variation were observed. The overall prevalence of rotavirus was 10.15% (13/128). RT-PCR targeting group specific VP6 gene confirmed Group A rotavirus in 10 out of 13 samples, while three samples remained un-groupable.

Entities:  

Keywords:  Bovine calves; Detection; Group A rotavirus; RNA-PAGE; RT-PCR

Year:  2011        PMID： 23637494      PMCID： PMC3550708          DOI： 10.1007/s13337-010-0017-9

Source DB:  PubMed          Journal:  Indian J Virol        ISSN： 0970-2822


  9 in total

1.  Rotavirus genotyping: keeping up with an evolving population of human rotaviruses.

Authors:  Miren Iturriza-Gómara; Gagandeep Kang; Jim Gray
Journal:  J Clin Virol       Date:  2004-12       Impact factor: 3.168

2.  Genomic diversity and prevalence of Rotavirus in cow and buffalo calves in northern India.

Authors:  S R Jindal; N K Maiti; M S Oberoi
Journal:  Rev Sci Tech       Date:  2000-12       Impact factor: 1.181

3.  Molecular epidemiology and subgroup determination of bovine group A rotaviruses associated with diarrhea in dairy and beef calves.

Authors:  K W Theil; C M McCloskey
Journal:  J Clin Microbiol       Date:  1989-01       Impact factor: 5.948

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels.

Authors:  A J Herring; N F Inglis; C K Ojeh; D R Snodgrass; J D Menzies
Journal:  J Clin Microbiol       Date:  1982-09       Impact factor: 5.948

6.  A study on the possibility of zoonotic infection in rotaviral diarrhoea among calves and buffalo calves in and around Kolkata, India.

Authors:  S M Nataraju; U K Chattopadhyay; T Krishnan
Journal:  Eur Rev Med Pharmacol Sci       Date:  2009 Jan-Feb       Impact factor: 3.507

7.  Prevalence of bovine group A rotavirus shedding among dairy calves in Ohio.

Authors:  A Lucchelli; S E Lance; P B Bartlett; G Y Miller; L J Saif
Journal:  Am J Vet Res       Date:  1992-02       Impact factor: 1.156

8.  Genotypification of bovine group A rotavirus in México.

Authors:  William A Rodríguez-Limas; Beatriz Flores-Samaniego; Germán de la Mora; Octavio T Ramírez; Laura A Palomares
Journal:  Vaccine       Date:  2009-06-24       Impact factor: 3.641

9.  Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California.

Authors:  J Chinsangaram; C E Schore; W Guterbock; L D Weaver; B I Osburn
Journal:  Comp Immunol Microbiol Infect Dis       Date:  1995-02       Impact factor: 2.268
  9 in total
  2 in total

Review 1.  Advances in Diagnostic Approaches for Viral Etiologies of Diarrhea: From the Lab to the Field.

Authors:  Yashpal Singh Malik; Atul Kumar Verma; Naveen Kumar; Nadia Touil; Kumaragurubaran Karthik; Ruchi Tiwari; Durlav Prasad Bora; Kuldeep Dhama; Souvik Ghosh; Maged Gomaa Hemida; Ahmed S Abdel-Moneim; Krisztián Bányai; Anastasia N Vlasova; Nobumichi Kobayashi; Raj Kumar Singh
Journal:  Front Microbiol       Date:  2019-09-13       Impact factor: 5.640

2.  Isolation and Characterization of Coronavirus and Rotavirus Associated with Calves in Central Part of Oromia, Ethiopia.

Authors:  Umer Seid; Fufa Dawo; Asamino Tesfaye; Munera Ahmednur
Journal:  Vet Med Int       Date:  2020-11-30
  2 in total

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