| Literature DB >> 7621673 |
J Chinsangaram1, C E Schore, W Guterbock, L D Weaver, B I Osburn.
Abstract
136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.Entities:
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Year: 1995 PMID： 7621673 PMCID： PMC7133876 DOI： 10.1016/0147-9571(95)98850-h
Source DB: PubMed Journal: Comp Immunol Microbiol Infect Dis ISSN： 0147-9571 Impact factor: 2.268