Literature DB >> 23623336

Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.

A Radu Aricescu1, Raymond J Owens.   

Abstract

Mammalian cells are rapidly becoming the system of choice for the production of recombinant glycoproteins for structural biology applications. Their use has enabled the structural investigation of a whole new set of targets including large, multi-domain and highly glycosylated eukaryotic cell surface receptors and their supra-molecular assemblies. We summarize the technical advances that have been made in mammalian expression technology and highlight some of the structural insights that have been obtained using these methods. Looking forward, it is clear that mammalian cell expression will provide exciting and unique opportunities for an integrative approach to the structural study of proteins, especially of human origin and medically relevant, by bridging the gap between the purified state and the cellular context.
Copyright © 2013 Elsevier Ltd. All rights reserved.

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Year:  2013        PMID： 23623336      PMCID： PMC4757734          DOI： 10.1016/j.sbi.2013.04.003

Source DB:  PubMed          Journal:  Curr Opin Struct Biol        ISSN： 0959-440X            Impact factor:   6.809


  89 in total

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Authors:  Arpana Dutta; Krishna Saxena; Harald Schwalbe; Judith Klein-Seetharaman
Journal:  Methods Mol Biol       Date:  2012

2.  An efficient platform for screening expression and crystallization of glycoproteins produced in human cells.

Authors:  Jeffrey E Lee; Marnie L Fusco; Erica Ollmann Saphire
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

3.  High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.

Authors:  Yves Durocher; Sylvie Perret; Amine Kamen
Journal:  Nucleic Acids Res       Date:  2002-01-15       Impact factor: 16.971

4.  Protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid.

Authors:  Nobumasa Hino; Yuko Okazaki; Takatsugu Kobayashi; Akiko Hayashi; Kensaku Sakamoto; Shigeyuki Yokoyama
Journal:  Nat Methods       Date:  2005-02-17       Impact factor: 28.547

5.  Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP.

Authors:  Benjamin Bishop; A Radu Aricescu; Karl Harlos; Chris A O'Callaghan; E Yvonne Jones; Christian Siebold
Journal:  Nat Struct Mol Biol       Date:  2009-06-28       Impact factor: 15.369

6.  High level expression of tissue inhibitor of metalloproteinases in Chinese hamster ovary cells using glutamine synthetase gene amplification.

Authors:  M I Cockett; C R Bebbington; G T Yarranton
Journal:  Biotechnology (N Y)       Date:  1990-07

7.  Genetic encoding of a bicyclo[6.1.0]nonyne-charged amino acid enables fast cellular protein imaging by metal-free ligation.

Authors:  Annika Borrmann; Sigrid Milles; Tilman Plass; Jan Dommerholt; Jorge M M Verkade; Manfred Wiessler; Carsten Schultz; Jan C M van Hest; Floris L van Delft; Edward A Lemke
Journal:  Chembiochem       Date:  2012-09-03       Impact factor: 3.164

8.  Proteoglycan-specific molecular switch for RPTPσ clustering and neuronal extension.

Authors:  Charlotte H Coles; Yingjie Shen; Alan P Tenney; Christian Siebold; Geoffrey C Sutton; Weixian Lu; John T Gallagher; E Yvonne Jones; John G Flanagan; A Radu Aricescu
Journal:  Science       Date:  2011-03-31       Impact factor: 47.728

9.  An extracellular steric seeding mechanism for Eph-ephrin signaling platform assembly.

Authors:  Elena Seiradake; Karl Harlos; Geoff Sutton; A Radu Aricescu; E Yvonne Jones
Journal:  Nat Struct Mol Biol       Date:  2010-03-14       Impact factor: 15.369

10.  Architecture and membrane interactions of the EGF receptor.

Authors:  Anton Arkhipov; Yibing Shan; Rahul Das; Nicholas F Endres; Michael P Eastwood; David E Wemmer; John Kuriyan; David E Shaw
Journal:  Cell       Date:  2013-01-31       Impact factor: 41.582
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  18 in total

1.  Baculovirus expression: old dog, new tricks.

Authors:  Imre Berger; Arnaud Poterszman
Journal:  Bioengineered       Date:  2015       Impact factor: 3.269

2.  Algal autolysate medium to label proteins for NMR in mammalian cells.

Authors:  Carmelo Fuccio; Enrico Luchinat; Letizia Barbieri; Sara Neri; Marco Fragai
Journal:  J Biomol NMR       Date:  2016-04-22       Impact factor: 2.835

Review 3.  Overview of the purification of recombinant proteins.

Authors:  Paul T Wingfield
Journal:  Curr Protoc Protein Sci       Date:  2015-04-01

4.  Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells.

Authors:  Sayan Chakraborty; Krittin Trihemasava; Guozhou Xu
Journal:  J Vis Exp       Date:  2018-08-20       Impact factor: 1.355

5.  Protein Crystallography from the Perspective of Technology Developments.

Authors:  Xiao-Dong Su; Heng Zhang; Thomas C Terwilliger; Anders Liljas; Junyu Xiao; Yuhui Dong
Journal:  Crystallogr Rev       Date:  2015       Impact factor: 2.467

6.  Mechanisms of inhibition and activation of extrasynaptic αβ GABAA receptors.

Authors:  Vikram Babu Kasaragod; Martin Mortensen; Steven W Hardwick; Ayla A Wahid; Valentina Dorovykh; Dimitri Y Chirgadze; Trevor G Smart; Paul S Miller
Journal:  Nature       Date:  2022-02-09       Impact factor: 49.962

7.  Recombinant protein expression for structural biology in HEK 293F suspension cells: a novel and accessible approach.

Authors:  Nicola Portolano; Peter J Watson; Louise Fairall; Christopher J Millard; Charles P Milano; Yun Song; Shaun M Cowley; John W R Schwabe
Journal:  J Vis Exp       Date:  2014-10-16       Impact factor: 1.355

8.  Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins.

Authors:  Jonathan Elegheert; Ester Behiels; Benjamin Bishop; Suzanne Scott; Rachel E Woolley; Samuel C Griffiths; Eamon F X Byrne; Veronica T Chang; David I Stuart; E Yvonne Jones; Christian Siebold; A Radu Aricescu
Journal:  Nat Protoc       Date:  2018-12       Impact factor: 13.491

9.  Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension.

Authors:  Silvia Faravelli; Matteo Campioni; Martina Palamini; Anselmo Canciani; Antonella Chiapparino; Federico Forneris
Journal:  Bio Protoc       Date:  2021-04-20

10.  Structural basis for GABAA receptor potentiation by neurosteroids.

Authors:  Paul S Miller; Suzanne Scott; Simonas Masiulis; Luigi De Colibus; Els Pardon; Jan Steyaert; A Radu Aricescu
Journal:  Nat Struct Mol Biol       Date:  2017-10-09       Impact factor: 15.369
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