Aflatoxin contamination in different fractions of rice from Pakistan and estimation of dietary intakes.

The purpose of this study was to determine the distribution of aflatoxins in rice milling fractions and to estimate dietary intakes. A total of 413 rice samples (paddy 58, parboiled 69, brown 84, white 93, and broken 109) were analyzed by HPLC with fluorescence detector. The results showed that 64 % paddy (16.35 ± 1.67 μg/kg), 38 % parboiled (14.20 ± 2.04 μg/kg), 33 % brown (9.85 ± 1.25 μg/kg), 42 % white (7.10 ± 1.39 μg/kg), and 50 % broken (8.5 ± 1.71 μg/kg) rice samples were contaminated with aflatoxins. It was found that paddy rice was most contaminated with aflatoxins while white rice was least contaminated. The percentage of samples exceeding EU maximum contents for total aflatoxins in rice (4 μg/kg) varied from 14 to 36 % when compared to the analyzed rice fractions. The total estimated amount of aflatoxin intake for average rice consumers ranged from 19.1 to 26.6 ng/kg body weight/day, much higher than the reference value of 1 ng/kg body weight/day. This is the first report discovering that rice is a major contributor to the dietary intake of aflatoxins in Pakistan.