Transcript profiling of the phytotoxic response of wheat to the Fusarium mycotoxin deoxynivalenol.

Deoxynivalenol (DON) is a trichothecene mycotoxin commonly produced by Fusarium graminearum and F. culmorum during infection of cereal plants, such as wheat and barley. This toxin is a fungal virulence factor that facilitates the development of Fusarium head blight (FHB) disease. Wheat cultivar (cv.) Remus is susceptible to DON; the toxin causes premature bleaching of spikelets and inhibits root growth. This study used custom-made wheat cDNA arrays to analyse the effect of DON on the transcriptome of heads of the toxin-sensitive wheat cv. Remus at both 4 and 24 h post-toxin treatment. DON-induced transcripts encoded an array of proteins collectively associated with a range of cellular functions, such as metabolite transformation and detoxification, the ubiquitin-proteasome proteolytic pathway, jasmonate biosynthesis and signalling, carbohydrate metabolism, and phenylpropanoid biosynthesis. This study is the first to demonstrate that the fungal virulence factor DON modulates jasmonate biosynthesis and signalling. It also highlights the fact that the toxin-mediated accumulation of transcripts associated with metabolite transformation and detoxification, proteolysis and phenylpropanoid accumulation is not unique to DON-resistant wheat genotypes. Therefore, the respective encoded proteins are likely part of the general wheat defence against DON. Comparative analysis of the results of this and other studies suggests that it is likely to be the rapidity and magnitude rather than the components of the response that are critical in determining resistance to DON and thus the spread of FHB disease in wheat heads.