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Literature DB >> 23589440

3D arrays for high throughput assay of cell migration and electrotaxis.

Sanjun Zhao¹, Runchi Gao, Peter N Devreotes, Alex Mogilner, Min Zhao.

Abstract

Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with different strains of cells, under various field strengths. These developments used Dictyostelium cells to illustrate possible applications and limitations.

Entities: Chemical Disease Species

Keywords: 3D matrices; array; assay; cell locomotion; electrotaxis; morphology

Mesh：

Substances：
Sepharose

Year: 2013 PMID： 23589440 PMCID： PMC3729600 DOI： 10.1002/cbin.10116

Source DB: PubMed Journal: Cell Biol Int ISSN： 1065-6995 Impact factor: 3.612

46 in total

1. Microfluidic device for studying cell migration in single or co-existing chemical gradients and electric fields.

Authors: Jing Li; Ling Zhu; Michael Zhang; Francis Lin
Journal: Biomicrofluidics Date: 2012-05-16 Impact factor: 2.800

2. Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis.

Authors: Muhammad H Zaman; Linda M Trapani; Alisha L Sieminski; Alisha Siemeski; Drew Mackellar; Haiyan Gong; Roger D Kamm; Alan Wells; Douglas A Lauffenburger; Paul Matsudaira
Journal: Proc Natl Acad Sci U S A Date: 2006-07-10 Impact factor: 11.205

Review 3. Microfluidic devices for studying chemotaxis and electrotaxis.

Authors: Jing Li; Francis Lin
Journal: Trends Cell Biol Date: 2011-06-12 Impact factor: 20.808

4. Elucidating the role of matrix stiffness in 3D cell migration and remodeling.

Authors: M Ehrbar; A Sala; P Lienemann; A Ranga; K Mosiewicz; A Bittermann; S C Rizzi; F E Weber; M P Lutolf
Journal: Biophys J Date: 2011-01-19 Impact factor: 4.033

5. Controlled architectural and chemotactic studies of 3D cell migration.

Authors: Prakriti Tayalia; Eric Mazur; David J Mooney
Journal: Biomaterials Date: 2011-01-14 Impact factor: 12.479

6. Human corneal epithelial cells reorient and migrate cathodally in a small applied electric field.

Authors: M Zhao; C D McCaig; A Agius-Fernandez; J V Forrester; K Araki-Sasaki
Journal: Curr Eye Res Date: 1997-10 Impact factor: 2.424

7. Effects of physiological electric fields on migration of human dermal fibroblasts.

Authors: Aihua Guo; Bing Song; Brian Reid; Yu Gu; John V Forrester; Colin A B Jahoda; Min Zhao
Journal: J Invest Dermatol Date: 2010-04-22 Impact factor: 8.551

8. Micro-electro-fluidic grids for nematodes: a lens-less, image-sensor-less approach for on-chip tracking of nematode locomotion.

Authors: Peng Liu; Richard J Martin; Liang Dong
Journal: Lab Chip Date: 2013-02-21 Impact factor: 6.799

9. HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment.

Authors: Britta Weigelt; Alvin T Lo; Catherine C Park; Joe W Gray; Mina J Bissell
Journal: Breast Cancer Res Treat Date: 2009-08-22 Impact factor: 4.872

3D arrays for high throughput assay of cell migration and electrotaxis.

1. Microfluidic device for studying cell migration in single or co-existing chemical gradients and electric fields.

2. Migration of tumor cells in 3D matrices is governed by matrix stiffness along with cell-matrix adhesion and proteolysis.

Review 3. Microfluidic devices for studying chemotaxis and electrotaxis.

4. Elucidating the role of matrix stiffness in 3D cell migration and remodeling.

5. Controlled architectural and chemotactic studies of 3D cell migration.

6. Human corneal epithelial cells reorient and migrate cathodally in a small applied electric field.

7. Effects of physiological electric fields on migration of human dermal fibroblasts.

8. Micro-electro-fluidic grids for nematodes: a lens-less, image-sensor-less approach for on-chip tracking of nematode locomotion.

9. HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment.

10. Multizone paper platform for 3D cell cultures.

1. Electrotaxis-on-Chip to Quantify Neutrophil Migration Towards Electrochemical Gradients.

Review 2. Three-dimensional culture systems in cancer research: Focus on tumor spheroid model.

3. Cellular microenvironment modulates the galvanotaxis of brain tumor initiating cells.

4. Electrotaxis of Glioblastoma and Medulloblastoma Spheroidal Aggregates.

5. Collagen Matrices Mediate Glioma Cell Migration Induced by an Electrical Signal.