Literature DB >> 23571295

Novel PCR assay for determining the genetic sex of mice.

L McFarlane1, V Truong, J S Palmer, D Wilhelm.   

Abstract

A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.
Copyright © 2013 S. Karger AG, Basel.

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Year:  2013        PMID： 23571295     DOI： 10.1159/000348677

Source DB:  PubMed          Journal:  Sex Dev        ISSN： 1661-5425            Impact factor:   1.824


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