Literature DB >> 28528577

Single-step PCR-based genetic sex determination of rat tissues and cells.

Pramod Dhakal1, Michael J Soares1.   

Abstract

The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.

Entities:  

Keywords:  fetal; rat; sex determination

Mesh：

Year:  2017        PMID： 28528577      PMCID： PMC5831150          DOI： 10.2144/000114548

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  19 in total

1.  Quick sex determination of mouse fetuses.

Authors:  J F Lambert; B O Benoit; G A Colvin; J Carlson; Y Delville; P J Quesenberry
Journal:  J Neurosci Methods       Date:  2000-02-15       Impact factor: 2.390

Review 2.  Epigenesis and plasticity of mouse trophoblast stem cells.

Authors:  Julie Prudhomme; Céline Morey
Journal:  Cell Mol Life Sci       Date:  2015-11-05       Impact factor: 9.261

3.  Sexing of mouse preimplantation embryos by detection of Y chromosome-specific sequences using polymerase chain reaction.

Authors:  T Kunieda; M Xian; E Kobayashi; T Imamichi; K Moriwaki; Y Toyoda
Journal:  Biol Reprod       Date:  1992-04       Impact factor: 4.285

Review 4.  Review: Sex and the human placenta: mediating differential strategies of fetal growth and survival.

Authors:  V L Clifton
Journal:  Placenta       Date:  2009-12-11       Impact factor: 3.481

Review 5.  Review: Sexual dimorphism in the formation, function and adaptation of the placenta.

Authors:  J I Kalisch-Smith; D G Simmons; H Dickinson; K M Moritz
Journal:  Placenta       Date:  2016-12-08       Impact factor: 3.481

6.  Policy: NIH to balance sex in cell and animal studies.

Authors:  Janine A Clayton; Francis S Collins
Journal:  Nature       Date:  2014-05-15       Impact factor: 49.962

7.  FGF4-dependent stem cells derived from rat blastocysts differentiate along the trophoblast lineage.

Authors:  Kazuo Asanoma; M A Karim Rumi; Lindsey N Kent; Damayanti Chakraborty; Stephen J Renaud; Norio Wake; Dong-Soo Lee; Kaiyu Kubota; Michael J Soares
Journal:  Dev Biol       Date:  2011-01-05       Impact factor: 3.582

8.  Trophoblast cell differentiation: establishment, characterization, and modulation of a rat trophoblast cell line expressing members of the placental prolactin family.

Authors:  T N Faria; M J Soares
Journal:  Endocrinology       Date:  1991-12       Impact factor: 4.736

Review 9.  Effect of preeclampsia on placental function: influence of sexual dimorphism, microRNA's and mitochondria.

Authors:  Leslie Myatt; Sribalasubashini Muralimanoharan; Alina Maloyan
Journal:  Adv Exp Med Biol       Date:  2014       Impact factor: 2.622

10.  Novel PCR assay for determining the genetic sex of mice.

Authors:  L McFarlane; V Truong; J S Palmer; D Wilhelm
Journal:  Sex Dev       Date:  2013-04-04       Impact factor: 1.824
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  6 in total

1.  Serotonin homeostasis in the materno-foetal interface at term: Role of transporters (SERT/SLC6A4 and OCT3/SLC22A3) and monoamine oxidase A (MAO-A) in uptake and degradation of serotonin by human and rat term placenta.

Authors:  Rona Karahoda; Hana Horackova; Petr Kastner; Andreas Matthios; Lukas Cerveny; Radim Kucera; Marian Kacerovsky; Jurjen Duintjer Tebbens; Alexandre Bonnin; Cilia Abad; Frantisek Staud
Journal:  Acta Physiol (Oxf)       Date:  2020-05-18       Impact factor: 6.311

2.  Gestational Iron Supplementation Improves Fetal Outcomes in a Rat Model of Prenatal Alcohol Exposure.

Authors:  Kaylee K Helfrich; Nipun Saini; Sze Ting Cecilia Kwan; Olivia C Rivera; Rachel Hodges; Susan M Smith
Journal:  Nutrients       Date:  2022-04-15       Impact factor: 6.706

3.  Comparison of rat fetal sex determination using placental gDNA and mRNA via qRT-PCR.

Authors:  Anthony L Su; Rita Loch-Caruso
Journal:  J Mol Biol Methods       Date:  2020-07-05

4.  Maternal Opioid Exposure Culminates in Perturbed Murine Neurodevelopment and Hyperactive Phenotype in Adolescence.

Authors:  Caitlin R Schlagal; Tiffany J Dunn; Pei Xu; Daniel E Felsing; Christina R Merritt; Sanjana Manja; Robert G Fox; Shelly A Buffington; George Saade; Kelly T Dineley; Yongjia Yu; Kathryn A Cunningham; Ping Wu
Journal:  Neuroscience       Date:  2021-03-31       Impact factor: 3.708

5.  Brown rats and house mice eavesdrop on each other's volatile sex pheromone components.

Authors:  Elana Varner; Hanna Jackson; Manveer Mahal; Stephen Takács; Regine Gries; Gerhard Gries
Journal:  Sci Rep       Date:  2020-10-19       Impact factor: 4.379

6.  Antenatal Endotoxin Impairs Lung Mechanics and Increases Sensitivity to Ventilator-Induced Lung Injury in Newborn Rat Pups.

Authors:  Erica W Mandell; Courtney Mattson; Gregory Seedorf; Sharon Ryan; Tania Gonzalez; Alison Wallbank; Elisa M Bye; Steven H Abman; Bradford J Smith
Journal:  Front Physiol       Date:  2021-01-13       Impact factor: 4.566
  6 in total

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