OBJECTIVE: To analyze the chemical composition and to evaluate the bioactive potential of hydroalocoholic extract of propolis. METHODS: Ethanol extract of propolis was analyzed by GC-MS, HPTLC and HPLC methods and in vitro antioxidant, anticholinesterase and cytotoxicity assay were performed. RESULTS: GC-MS analysis revealed the presence of fatty acids, alcohols, and quercetin. Quercetin was identified and quantified by HPTLC and HPLC methods. Dose dependent DPPH and hydroxyl radical scavenging activity of hydroalcoholic extract of propolis was calculated as 16.20 and 34.33 µg/mL respectively. Inhibition of lipid peroxidation was significant and the IC50 value was calculated as 55.56µg/mL. Anticholinesterase activity was less observed. The cytotoxic activity against both breast (MCF-7) and lung cancer (A543) cell lines were significant and the IC50 value was calculated as 10 and 13 µg/mL respectively. CONCLUSIONS: These findings showed that bioactive compounds present in propolis will alleviate many diseases and can be used for better human health.
OBJECTIVE: To analyze the chemical composition and to evaluate the bioactive potential of hydroalocoholic extract of propolis. METHODS:Ethanol extract of propolis was analyzed by GC-MS, HPTLC and HPLC methods and in vitro antioxidant, anticholinesterase and cytotoxicity assay were performed. RESULTS: GC-MS analysis revealed the presence of fatty acids, alcohols, and quercetin. Quercetin was identified and quantified by HPTLC and HPLC methods. Dose dependent DPPH and hydroxyl radical scavenging activity of hydroalcoholic extract of propolis was calculated as 16.20 and 34.33 µg/mL respectively. Inhibition of lipid peroxidation was significant and the IC50 value was calculated as 55.56µg/mL. Anticholinesterase activity was less observed. The cytotoxic activity against both breast (MCF-7) and lung cancer (A543) cell lines were significant and the IC50 value was calculated as 10 and 13 µg/mL respectively. CONCLUSIONS: These findings showed that bioactive compounds present in propolis will alleviate many diseases and can be used for better human health.
Authors: Hongzhen Li; Aneesh Kapur; Jesse X Yang; Shiv Srivastava; David G McLeod; Julio F Paredes-Guzman; Andreas Daugsch; Yong K Park; Johng S Rhim Journal: Int J Oncol Date: 2007-09 Impact factor: 5.650
Authors: Jadriane de Almeida Xavier; Iara Barros Valentim; Fabiana O S Camatari; Alberto M M de Almeida; Henrique Fonseca Goulart; Jamylle Nunes de Souza Ferro; Emiliano de Oliveira Barreto; Bruno Coelho Cavalcanti; Carla B G Bottoli; Marília Oliveira Fonseca Goulart Journal: Pharm Biol Date: 2017-12 Impact factor: 3.503