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Literature DB >> 23545650

Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex.

Peter Thomas Naumann¹, Charles T Lauhon, Ralf Ficner.

Abstract

The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 Å.

Entities: Chemical Gene Species

Keywords: 4-thiouridine; protein–RNA complex

Mesh：

Substances：
Thiouridine
RNA
Ligases

Year: 2013 PMID： 23545650 PMCID： PMC3614169 DOI： 10.1107/S1744309113004077

Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091

30 in total

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Authors: Delin You; Tiegang Xu; Fen Yao; Xiufen Zhou; Zixin Deng
Journal: Chembiochem Date: 2008-08-11 Impact factor: 3.164

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Authors: J Rozenski; P F Crain; J A McCloskey
Journal: Nucleic Acids Res Date: 1999-01-01 Impact factor: 16.971

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Authors: E G Mueller; C J Buck; P M Palenchar; L E Barnhart; J L Paulson
Journal: Nucleic Acids Res Date: 1998-06-01 Impact factor: 16.971

4. Solvent content of protein crystals.

Authors: B W Matthews
Journal: J Mol Biol Date: 1968-04-28 Impact factor: 5.469

5. Deduced RNA binding mechanism of ThiI based on structural and binding analyses of a minimal RNA ligand.

Authors: Yoshikazu Tanaka; Shiori Yamagata; Yu Kitago; Yoko Yamada; Sarin Chimnaronk; Min Yao; Isao Tanaka
Journal: RNA Date: 2009-06-09 Impact factor: 4.942

6. Substrate specificity for 4-thiouridine modification in Escherichia coli.

Authors: Charles T Lauhon; Whitney M Erwin; Giangthy N Ton
Journal: J Biol Chem Date: 2004-03-22 Impact factor: 5.157

7. An introduction to data reduction: space-group determination, scaling and intensity statistics.

Authors: Philip R Evans
Journal: Acta Crystallogr D Biol Crystallogr Date: 2011-03-18

8. Overview of the CCP4 suite and current developments.

Authors: Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal: Acta Crystallogr D Biol Crystallogr Date: 2011-03-18

9. MODOMICS: a database of RNA modification pathways--2013 update.

Authors: Magdalena A Machnicka; Kaja Milanowska; Okan Osman Oglou; Elzbieta Purta; Malgorzata Kurkowska; Anna Olchowik; Witold Januszewski; Sebastian Kalinowski; Stanislaw Dunin-Horkawicz; Kristian M Rother; Mark Helm; Janusz M Bujnicki; Henri Grosjean
Journal: Nucleic Acids Res Date: 2012-10-30 Impact factor: 16.971