Literature DB >> 234862

The case for assigning a value of approximately 4 to pKa-i of the essential histidine-cysteine interactive systems of papain, bromelain and ficin.

M Shipton, M P Kierstan, J P Malthouse, T Stuchbury, K Brocklehurst.   

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Year:  1975        PMID： 234862     DOI： 10.1016/0014-5793(75)80529-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


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  14 in total

1.  PH-dependence of the steady-state rate of a two-step enzymic reaction.

Authors:  K Brocklehurst; H B Dixon
Journal:  Biochem J       Date:  1976-04-01       Impact factor: 3.857

2.  The unreliability of estimates of group dissociation constants.

Authors:  H B Dixon
Journal:  Biochem J       Date:  1976-03-01       Impact factor: 3.857

3.  A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety.

Authors:  T Stuchbury; M Shipton; R Norris; J P Malthouse; K Brocklehurst; J A Herbert; H Suschitzky
Journal:  Biochem J       Date:  1975-11       Impact factor: 3.857

4.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

5.  Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.

Authors:  D Kowlessur; C M Topham; E W Thomas; M O'Driscoll; W Templeton; K Brocklehurst
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

6.  Benzofuroxan as a thiol-specific reactivity probe. Kinetics of its reactions with papain, ficin, bromelain and low-molecular-weight thiols.

Authors:  M Shipton; K Brocklehurst
Journal:  Biochem J       Date:  1977-12-01       Impact factor: 3.857

7.  Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu.

Authors:  G W Mellor; M Patel; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-08-15       Impact factor: 3.857

8.  Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism.

Authors:  K Brocklehurst; J P Malthouse
Journal:  Biochem J       Date:  1978-11-01       Impact factor: 3.857

9.  Cysteine pKa depression by a protonated glutamic acid in human DJ-1.

Authors:  Anna C Witt; Mahadevan Lakshminarasimhan; Benjamin C Remington; Sahar Hasim; Edwin Pozharski; Mark A Wilson
Journal:  Biochemistry       Date:  2008-06-21       Impact factor: 3.162

10.  Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe.

Authors:  G W Mellor; E W Thomas; C M Topham; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857
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