Intramolecular zero-quantum-coherence 2D NMR spectroscopy of lipids in the human breast at 7 T.

PURPOSE: Intramolecular zero-quantum-coherences (ZQCs) are intrinsically insensitive toward magnetic field inhomogeneity. This fact is used to quantify and characterize lipid signals in the human breast at 7 T despite the presence of severe magnetic field inhomogeneity caused by water-lipid susceptibility boundaries.
METHODS: A novel 3D localized 2D ZQC method is presented. The combination of cardiac/respiratory triggering and post-acquisition navigator echo correction provides high-quality 2D NMR spectra in vivo.
RESULTS: The lipid profile of the human breast could be quantified by 2D ZQC NMR in 100% of the subjects despite a wide range of magnetic field homogeneity. With conventional 1D (1)H MRS, the magnetic field homogeneity was only adequate in 60% of the subjects. The results from 2D ZQC NMR and 1D NMR are in good correspondence, both in vitro and in vivo.
CONCLUSION: It has been demonstrated that high quality and quantitative 2D ZQC NMR spectra can be acquired from human breast tissue at 7 T. While the simplicity and sensitivity of 1D MRS are preferable when the magnetic field homogeneity is adequate, the 2D ZQC method provides a viable alternative in cases where this requirement cannot be met.