PROBLEM: This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation. METHOD OF STUDY: We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as "ICSI-like", and (iii) with MSOME. RESULTS: The balanced translocation rates did not differ significantly (p=0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p=0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %). CONCLUSION: For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.
PROBLEM: This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation. METHOD OF STUDY: We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as "ICSI-like", and (iii) with MSOME. RESULTS: The balanced translocation rates did not differ significantly (p=0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p=0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %). CONCLUSION: For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.
Authors: Pierre Vanderzwalmen; Antje Hiemer; Paul Rubner; Magnus Bach; Anton Neyer; Astrid Stecher; Petr Uher; Martin Zintz; Bernard Lejeune; Sabine Vanderzwalmen; Guy Cassuto; Nicolas H Zech Journal: Reprod Biomed Online Date: 2008-11 Impact factor: 3.828
Authors: J G Franco; R L R Baruffi; A L Mauri; C G Petersen; J B A Oliveira; L Vagnini Journal: Reprod Biomed Online Date: 2008-07 Impact factor: 3.828
Authors: Mohamed Hassen Chelli; Martine Albert; Pierre F Ray; Bruno Guthauser; Vincent Izard; Ibrahim Hammoud; Jacqueline Selva; François Vialard Journal: Fertil Steril Date: 2008-12-04 Impact factor: 7.329
Authors: E Mateu-Brull; L Rodrigo; V Peinado; A Mercader; I Campos-Galindo; F Bronet; S García-Herrero; M Florensa; M Milán; C Rubio Journal: J Assist Reprod Genet Date: 2019-11-06 Impact factor: 3.412
Authors: Florence Boitrelle; Bruno Guthauser; Laura Alter; Marc Bailly; Robert Wainer; François Vialard; Martine Albert; Jacqueline Selva Journal: Basic Clin Androl Date: 2013-08-29