Literature DB >> 23436370

Immunoaffinity purification of protein complexes from Mammalian cells.

Chieri Tomomori-Sato1, Shigeo Sato, Ronald C Conaway, Joan W Conaway.   

Abstract

In this chapter, we describe a purification scheme designed to isolate multisubunit protein complexes gently and quickly from crude extracts of mammalian cells using immunoaffinity purification of epitope tagged proteins and the multisubunit complexes with which they associate. As an example we describe isolation of the mammalian Mediator complex from HeLa S3 cells.

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Year:  2013        PMID： 23436370      PMCID： PMC3693849          DOI： 10.1007/978-1-62703-284-1_22

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

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Journal:  J Biochem Biophys Methods       Date:  2001-10-30

Review 2.  Epitope tagging.

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Journal:  Annu Rev Genet       Date:  1998       Impact factor: 16.830

3.  Human mediator subunit MED26 functions as a docking site for transcription elongation factors.

Authors:  Hidehisa Takahashi; Tari J Parmely; Shigeo Sato; Chieri Tomomori-Sato; Charles A S Banks; Stephanie E Kong; Henrietta Szutorisz; Selene K Swanson; Skylar Martin-Brown; Michael P Washburn; Laurence Florens; Chris W Seidel; Chengqi Lin; Edwin R Smith; Ali Shilatifard; Ronald C Conaway; Joan W Conaway
Journal:  Cell       Date:  2011-07-08       Impact factor: 41.582

4.  DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

Authors:  J L Manley; A Fire; A Cano; P A Sharp; M L Gefter
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

5.  Epitope-tagged ubiquitin. A new probe for analyzing ubiquitin function.

Authors:  M J Ellison; M Hochstrasser
Journal:  J Biol Chem       Date:  1991-11-05       Impact factor: 5.157

6.  Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics.

Authors:  J A Southern; D F Young; F Heaney; W K Baumgärtner; R E Randall
Journal:  J Gen Virol       Date:  1991-07       Impact factor: 3.891

7.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

8.  A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.

Authors:  Shigeo Sato; Chieri Tomomori-Sato; Tari J Parmely; Laurence Florens; Boris Zybailov; Selene K Swanson; Charles A S Banks; Jingji Jin; Yong Cai; Michael P Washburn; Joan Weliky Conaway; Ronald C Conaway
Journal:  Mol Cell       Date:  2004-06-04       Impact factor: 17.970

9.  Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.

Authors:  J Field; J Nikawa; D Broek; B MacDonald; L Rodgers; I A Wilson; R A Lerner; M Wigler
Journal:  Mol Cell Biol       Date:  1988-05       Impact factor: 4.272
  9 in total
  9 in total

1.  Role for human mediator subunit MED25 in recruitment of mediator to promoters by endoplasmic reticulum stress-responsive transcription factor ATF6α.

Authors:  Dotan Sela; Juliana J Conkright; Lu Chen; Joshua Gilmore; Michael P Washburn; Laurence Florens; Ronald C Conaway; Joan Weliky Conaway
Journal:  J Biol Chem       Date:  2013-07-17       Impact factor: 5.157

2.  A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction.

Authors:  Somnath Mukherjee; Marcin Ura; Robert J Hoey; Anthony A Kossiakoff
Journal:  J Mol Biol       Date:  2015-07-06       Impact factor: 5.469

3.  Role for the MED21-MED7 Hinge in Assembly of the Mediator-RNA Polymerase II Holoenzyme.

Authors:  Shigeo Sato; Chieri Tomomori-Sato; Kuang-Lei Tsai; Xiaodi Yu; Mihaela Sardiu; Anita Saraf; Michael P Washburn; Laurence Florens; Francisco J Asturias; Ronald C Conaway; Joan W Conaway
Journal:  J Biol Chem       Date:  2016-11-07       Impact factor: 5.157

4.  Immunoaffinity Purification of Epitope-Tagged DNA Repair Complexes from Human Cells.

Authors:  Brittany A Townley; Jennifer M Soll; Nima Mosammaparast
Journal:  Methods Mol Biol       Date:  2022

5.  DNA methylation on N(6)-adenine in mammalian embryonic stem cells.

Authors:  Tao P Wu; Tao Wang; Matthew G Seetin; Yongquan Lai; Shijia Zhu; Kaixuan Lin; Yifei Liu; Stephanie D Byrum; Samuel G Mackintosh; Mei Zhong; Alan Tackett; Guilin Wang; Lawrence S Hon; Gang Fang; James A Swenberg; Andrew Z Xiao
Journal:  Nature       Date:  2016-03-30       Impact factor: 49.962

6.  mTORC2 modulates feedback regulation of p38 MAPK activity via DUSP10/MKP5 to confer differential responses to PP242 in glioblastoma.

Authors:  Angelica Benavides-Serrato; Lauren Anderson; Brent Holmes; Cheri Cloninger; Nicholas Artinian; Tariq Bashir; Joseph Gera
Journal:  Genes Cancer       Date:  2014-11

7.   De novo isolation of antibodies with pH-dependent binding properties.

Authors:  Pauline Bonvin; Sophie Venet; Gaëlle Fontaine; Ulla Ravn; Franck Gueneau; Marie Kosco-Vilbois; Amanda Ei Proudfoot; Nicolas Fischer
Journal:  MAbs       Date:  2015       Impact factor: 5.857

8.  CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts.

Authors:  Melvin Noe Gonzalez; Shigeo Sato; Chieri Tomomori-Sato; Joan W Conaway; Ronald C Conaway
Journal:  Nat Commun       Date:  2018-08-23       Impact factor: 14.919

9.  Examining the Protein Interactome and Subcellular Localization of RNase J2 Complexes in Streptococcus mutans.

Authors:  Rong Mu; Pushkar Shinde; Zhengzhong Zou; Jens Kreth; Justin Merritt
Journal:  Front Microbiol       Date:  2019-09-18       Impact factor: 5.640
  9 in total

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