| Literature DB >> 23413188 |
Jan Kern1, Roberto Alonso-Mori, Rosalie Tran, Johan Hattne, Richard J Gildea, Nathaniel Echols, Carina Glöckner, Julia Hellmich, Hartawan Laksmono, Raymond G Sierra, Benedikt Lassalle-Kaiser, Sergey Koroidov, Alyssa Lampe, Guangye Han, Sheraz Gul, Dörte Difiore, Despina Milathianaki, Alan R Fry, Alan Miahnahri, Donald W Schafer, Marc Messerschmidt, M Marvin Seibert, Jason E Koglin, Dimosthenis Sokaras, Tsu-Chien Weng, Jonas Sellberg, Matthew J Latimer, Ralf W Grosse-Kunstleve, Petrus H Zwart, William E White, Pieter Glatzel, Paul D Adams, Michael J Bogan, Garth J Williams, Sébastien Boutet, Johannes Messinger, Athina Zouni, Nicholas K Sauter, Vittal K Yachandra, Uwe Bergmann, Junko Yano.
Abstract
Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S1) and the first illuminated state (S2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.Entities:
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Year: 2013 PMID： 23413188 PMCID： PMC3732582 DOI： 10.1126/science.1234273
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728