Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Sorting short fragments of single-stranded DNA with an evolving electric double layer.

Literature DB >> 23356906

Sorting short fragments of single-stranded DNA with an evolving electric double layer.

Jiamin Wu¹, Shuang-Liang Zhao, Lizeng Gao, Jianzhong Wu, Di Gao.

Abstract

We demonstrate a new procedure for separation of single-stranded DNA (ssDNA) fragments that are anchored to the surface of a gold electrode by end hybridization. The new separation procedure takes advantage of the strong yet evolving nonuniform electric field near the gold surface in contact with a buffer solution gradually being diluted with deionized water. Separation of short ssDNA fragments is demonstrated by monitoring the DNA at the gold surface with in situ fluorescence measurement. The experimental results can be rationalized with a simple theoretical model of electric double layer that relates the strength of the surface pulling force to the ionic concentration of the changing buffer solution.

Entities: Chemical Species

Mesh：

Substances：

Year: 2013 PMID： 23356906 PMCID： PMC3624732 DOI： 10.1021/jp3096715

Source DB: PubMed Journal: J Phys Chem B ISSN： 1520-5207 Impact factor: 2.991

29 in total

1. Electrostatic surface plasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches.

Authors: R J Heaton; A W Peterson; R M Georgiadis
Journal: Proc Natl Acad Sci U S A Date: 2001-03-20 Impact factor: 11.205

Sorting short fragments of single-stranded DNA with an evolving electric double layer.

1. Electrostatic surface plasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches.

2. Direct mechanical measurements of the elasticity of single DNA molecules by using magnetic beads.

3. Separation of single-stranded DNA fragments at a 10-nucleotide resolution by stretching in microfluidic channels.

4. Density functional study of the electric double layer formed by a high density electrolyte.

5. Effective charge and free energy of DNA inside an ion channel.

6. Ionic effects in collapse of polyelectrolyte brushes.

7. Platform for in situ real-time measurement of protein-induced conformational changes of DNA.

8. Nucleic acid helix stability: effects of salt concentration, cation valence and size, and chain length.

9. Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control.

10. Counter-ion condensation and system dimensionality.

1. Melting dynamics of short dsDNA chains in saline solutions.