Literature DB >> 2335353

Rapid analysis of mouse-hamster hybrid cell lines by in situ hybridization.

A L Boyle1, P Lichter, D C Ward.   

Abstract

In situ hybridization techniques for analyzing the murine DNA complement of mouse-hamster hybrid cells are described. Total genomic mouse DNA is labeled with biotin and hybridized without suppression to metaphase spreads from a mouse-hamster hybrid line containing the mouse fusion chromosome X12. Detection via fluorochrome-conjugated avidin reveals mouse chromosomal DNA with high sensitivity and permits the identification of both normal and aberrant murine chromosomes. Conversely, biotinylated total genomic DNA from a hybrid line can be used as a probe on normal mouse metaphase spreads if suppression techniques are employed, facilitating the analysis of mouse chromosomes present in the hybrid line.

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Year:  1990        PMID： 2335353     DOI： 10.1016/0888-7543(90)90529-4

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  9 in total

1.  Microscopy and image analysis.

Authors:  George McNamara; Michael J Difilippantonio; Thomas Ried
Journal:  Curr Protoc Hum Genet       Date:  2005-08

2.  Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.

Authors:  A L Boyle; S G Ballard; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

3.  Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization.

Authors:  Thomas Cremer; Christoph Cremer; Peter Lichter
Journal:  Hum Genet       Date:  2014-02-07       Impact factor: 4.132

4.  Fluorescence in situ hybridization with Alu and L1 polymerase chain reaction probes for rapid characterization of human chromosomes in hybrid cell lines.

Authors:  P Lichter; S A Ledbetter; D H Ledbetter; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

5.  In situ hybridization of PCR amplified inter-Alu sequences from a hybrid cell line.

Authors:  C Desmaze; J Zucman; O Delattre; G Thomas; A Aurias
Journal:  Hum Genet       Date:  1992-03       Impact factor: 4.132

6.  Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers.

Authors:  C Lengauer; H Riethman; T Cremer
Journal:  Hum Genet       Date:  1990-11       Impact factor: 4.132

Review 7.  Advanced molecular cytogenetics in human and mouse.

Authors:  Kathleen Dorritie; Cristina Montagna; Michael J Difilippantonio; Thomas Ried
Journal:  Expert Rev Mol Diagn       Date:  2004-09       Impact factor: 5.225

8.  Detection of amplified DNA sequences by reverse chromosome painting using genomic tumor DNA as probe.

Authors:  S Joos; H Scherthan; M R Speicher; J Schlegel; T Cremer; P Lichter
Journal:  Hum Genet       Date:  1993-02       Impact factor: 4.132

9.  Ewing's tumor X mouse hybrids expressing the MIC2 antigen: analyses using fluorescence CDD-banding and non-isotopic ISH.

Authors:  M Dworzak; C Stock; S Strehl; H Gadner; P F Ambros
Journal:  Hum Genet       Date:  1992-01       Impact factor: 4.132
  9 in total

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