Literature DB >> 23345045

Long wavelength fluorescence ratiometric zinc biosensor.

Hui Hui Zeng1, Evgenia G Matveeva, Andrea K Stoddard, Carol A Fierke, Richard B Thompson.   

Abstract

A protein-based emission ratiometric fluorescence biosensor is described that exhibits sensitivity to free zinc ion in solution down to picomolar concentrations. Ratiometric measurements are widely used to assure accurate quantitation, and emission ratios are preferred for laser scanning microscopes such as confocal fluorescence microscopes. The relatively long emission wavelengths used are well suited to studies in tissues and other matrices which exhibit significant fluorescence background, and the apo-carbonic anhydrase moiety recognizes zinc ion with high and controllable specificity.

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Year:  2013        PMID: 23345045      PMCID: PMC3656977          DOI: 10.1007/s10895-013-1161-6

Source DB:  PubMed          Journal:  J Fluoresc        ISSN: 1053-0509            Impact factor:   2.217


  26 in total

1.  Enhanced fluorescence resonance energy transfer between spectral variants of green fluorescent protein through zinc-site engineering.

Authors:  K K Jensen; L Martini; T W Schwartz
Journal:  Biochemistry       Date:  2001-01-30       Impact factor: 3.162

2.  Variation of linker length in ratiometric fluorescent sensor proteins allows rational tuning of Zn(II) affinity in the picomolar to femtomolar range.

Authors:  Elisabeth M W M van Dongen; Toon H Evers; Linda M Dekkers; E W Meijer; Leo W J Klomp; Maarten Merkx
Journal:  J Am Chem Soc       Date:  2007-03-03       Impact factor: 15.419

3.  Quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion.

Authors:  Bryan J McCranor; Rebecca A Bozym; Michele I Vitolo; Carol A Fierke; Linda Bambrick; Brian M Polster; Gary Fiskum; Richard B Thompson
Journal:  J Bioenerg Biomembr       Date:  2012-03-20       Impact factor: 2.945

4.  A quinoline fluorescence method for visualizing and assaying the histochemically reactive zinc (bouton zinc) in the brain.

Authors:  C J Frederickson; E J Kasarskis; D Ringo; R E Frederickson
Journal:  J Neurosci Methods       Date:  1987-06       Impact factor: 2.390

5.  Expanded dynamic range of free zinc ion determination by fluorescence anisotropy.

Authors:  R B Thompson; B P Maliwal; C A Fierke
Journal:  Anal Chem       Date:  1998-05-01       Impact factor: 6.986

6.  In vivo protein transduction: delivery of a biologically active protein into the mouse.

Authors:  S R Schwarze; A Ho; A Vocero-Akbani; S F Dowdy
Journal:  Science       Date:  1999-09-03       Impact factor: 47.728

7.  Development of a ratiometric fluorescent zinc ion probe in near-infrared region, based on tricarbocyanine chromophore.

Authors:  Kazuki Kiyose; Hirotatsu Kojima; Yasuteru Urano; Tetsuo Nagano
Journal:  J Am Chem Soc       Date:  2006-05-24       Impact factor: 15.419

8.  Organelle-specific zinc detection using zinpyr-labeled fusion proteins in live cells.

Authors:  Elisa Tomat; Elizabeth M Nolan; Jacek Jaworski; Stephen J Lippard
Journal:  J Am Chem Soc       Date:  2008-11-26       Impact factor: 15.419

9.  Reversal of the hydrogen bond to zinc ligand histidine-119 dramatically diminishes catalysis and enhances metal equilibration kinetics in carbonic anhydrase II.

Authors:  C C Huang; C A Lesburg; L L Kiefer; C A Fierke; D W Christianson
Journal:  Biochemistry       Date:  1996-03-19       Impact factor: 3.162

10.  Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors.

Authors:  Yan Qin; Philip J Dittmer; J Genevieve Park; Katarina B Jansen; Amy E Palmer
Journal:  Proc Natl Acad Sci U S A       Date:  2011-04-18       Impact factor: 11.205

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  1 in total

Review 1.  Fluorescent sensors for measuring metal ions in living systems.

Authors:  Kyle P Carter; Alexandra M Young; Amy E Palmer
Journal:  Chem Rev       Date:  2014-03-03       Impact factor: 60.622

  1 in total

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