| Literature DB >> 23341629 |
Stephen Lumayag1, Caroline E Haldin, Nicola J Corbett, Karl J Wahlin, Colleen Cowan, Sanja Turturro, Peter E Larsen, Beatrix Kovacs, P Dane Witmer, David Valle, Donald J Zack, Daniel A Nicholson, Shunbin Xu.
Abstract
The microRNA-183/96/182 cluster is highly expressed in the retina and other sensory organs. To uncover its in vivo functions in the retina, we generated a knockout mouse model, designated "miR-183C(GT/GT)," using a gene-trap embryonic stem cell clone. We provide evidence that inactivation of the cluster results in early-onset and progressive synaptic defects of the photoreceptors, leading to abnormalities of scotopic and photopic electroretinograms with decreased b-wave amplitude as the primary defect and progressive retinal degeneration. In addition, inactivation of the miR-183/96/182 cluster resulted in global changes in retinal gene expression, with enrichment of genes important for synaptogenesis, synaptic transmission, photoreceptor morphogenesis, and phototransduction, suggesting that the miR-183/96/182 cluster plays important roles in postnatal functional differentiation and synaptic connectivity of photoreceptors.Entities:
Mesh：
Substances：
Year: 2013 PMID： 23341629 PMCID： PMC3568372 DOI： 10.1073/pnas.1212655110
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205