Literature DB >> 23325687

An N-terminal amphipathic helix in dengue virus nonstructural protein 4A mediates oligomerization and is essential for replication.

Omer Stern1, Yu-Fu Hung, Olga Valdau, Yakey Yaffe, Eva Harris, Silke Hoffmann, Dieter Willbold, Ella H Sklan.   

Abstract

Dengue virus (DENV) causes dengue fever, a major health concern worldwide. We identified an amphipathic helix (AH) in the N-terminal region of the viral nonstructural protein 4A (NS4A). Disruption of its amphipathic nature using mutagenesis reduced homo-oligomerization and abolished viral replication. These data emphasize the significance of NS4A in the life cycle of the dengue virus and demarcate it as a target for the design of novel antiviral therapy.

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Year:  2013        PMID： 23325687      PMCID： PMC3624192          DOI： 10.1128/JVI.01900-12

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  23 in total

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Authors:  M A Cooper; A Hansson; S Löfås; D H Williams
Journal:  Anal Biochem       Date:  2000-01-15       Impact factor: 3.365

Review 2.  Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy.

Authors:  A K Kenworthy
Journal:  Methods       Date:  2001-07       Impact factor: 3.608

3.  Expression and purification of enzymatically active recombinant RNA-dependent RNA polymerase (NS5) of the flavivirus Kunjin.

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Journal:  J Virol Methods       Date:  2001-03       Impact factor: 2.014

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Journal:  Science       Date:  1989-08-04       Impact factor: 47.728

5.  A simple method for site-directed mutagenesis using the polymerase chain reaction.

Authors:  A Hemsley; N Arnheim; M D Toney; G Cortopassi; D J Galas
Journal:  Nucleic Acids Res       Date:  1989-08-25       Impact factor: 16.971

6.  Replication-deficient vaccinia virus encoding bacteriophage T7 RNA polymerase for transient gene expression in mammalian cells.

Authors:  L S Wyatt; B Moss; S Rozenblatt
Journal:  Virology       Date:  1995-06-20       Impact factor: 3.616

7.  Cleavage at a novel site in the NS4A region by the yellow fever virus NS2B-3 proteinase is a prerequisite for processing at the downstream 4A/4B signalase site.

Authors:  C Lin; S M Amberg; T J Chambers; C M Rice
Journal:  J Virol       Date:  1993-04       Impact factor: 5.103

8.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

Authors:  J D Thompson; D G Higgins; T J Gibson
Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

9.  The MARVEL transmembrane motif of occludin mediates oligomerization and targeting to the basolateral surface in epithelia.

Authors:  Yakey Yaffe; Jeanne Shepshelovitch; Inbar Nevo-Yassaf; Adva Yeheskel; Hedva Shmerling; Joanna M Kwiatek; Katharina Gaus; Metsada Pasmanik-Chor; Koret Hirschberg
Journal:  J Cell Sci       Date:  2012-04-03       Impact factor: 5.285

10.  Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication.

Authors:  Menashe Elazar; Kwang Ho Cheong; Ping Liu; Harry B Greenberg; Charles M Rice; Jeffrey S Glenn
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103
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  27 in total

Review 1.  Flavivirus RNA synthesis in vitro.

Authors:  Radhakrishnan Padmanabhan; Ratree Takhampunya; Tadahisa Teramoto; Kyung H Choi
Journal:  Methods       Date:  2015-08-10       Impact factor: 3.608

2.  Determinants of Dengue Virus NS4A Protein Oligomerization.

Authors:  Chia Min Lee; Xuping Xie; Jing Zou; Shi-Hua Li; Michelle Yue Qi Lee; Hongping Dong; Cheng-Feng Qin; Congbao Kang; Pei-Yong Shi
Journal:  J Virol       Date:  2015-04-01       Impact factor: 5.103

Review 3.  Biochemistry and Molecular Biology of Flaviviruses.

Authors:  Nicholas J Barrows; Rafael K Campos; Kuo-Chieh Liao; K Reddisiva Prasanth; Ruben Soto-Acosta; Shih-Chia Yeh; Geraldine Schott-Lerner; Julien Pompon; October M Sessions; Shelton S Bradrick; Mariano A Garcia-Blanco
Journal:  Chem Rev       Date:  2018-04-13       Impact factor: 60.622

4.  Genome-Wide Mutagenesis of Dengue Virus Reveals Plasticity of the NS1 Protein and Enables Generation of Infectious Tagged Reporter Viruses.

Authors:  Nicholas S Eyre; Stephen M Johnson; Auda A Eltahla; Maria Aloi; Amanda L Aloia; Christopher A McDevitt; Rowena A Bull; Michael R Beard
Journal:  J Virol       Date:  2017-11-14       Impact factor: 5.103

5.  Characterization of dengue virus NS4A and NS4B protein interaction.

Authors:  Jing Zou; Xuping Xie; Qing-Yin Wang; Hongping Dong; Michelle Yueqi Lee; Congbao Kang; Zhiming Yuan; Pei-Yong Shi
Journal:  J Virol       Date:  2015-01-07       Impact factor: 5.103

6.  A CRISPR Activation Screen Identifies Genes That Protect against Zika Virus Infection.

Authors:  Anna Dukhovny; Kevin Lamkiewicz; Qian Chen; Markus Fricke; Nabila Jabrane-Ferrat; Manja Marz; Jae U Jung; Ella H Sklan
Journal:  J Virol       Date:  2019-07-30       Impact factor: 5.103

Review 7.  Coupling of replication and assembly in flaviviruses.

Authors:  Swapna Apte-Sengupta; Devika Sirohi; Richard J Kuhn
Journal:  Curr Opin Virol       Date:  2014-10-18       Impact factor: 7.090

8.  Determinants in Nonstructural Protein 4A of Dengue Virus Required for RNA Replication and Replication Organelle Biogenesis.

Authors:  Mirko Cortese; Klaas Mulder; Laurent Chatel-Chaix; Pietro Scaturro; Berati Cerikan; Anna Plaszczyca; Uta Haselmann; Marie Bartenschlager; Christopher J Neufeldt; Ralf Bartenschlager
Journal:  J Virol       Date:  2021-08-11       Impact factor: 5.103

9.  Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.

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Journal:  J Virol       Date:  2013-11-27       Impact factor: 5.103

10.  Chaperone-Assisted Protein Folding Is Critical for Yellow Fever Virus NS3/4A Cleavage and Replication.

Authors:  Leonia Bozzacco; Zhigang Yi; Ursula Andreo; Claire R Conklin; Melody M H Li; Charles M Rice; Margaret R MacDonald
Journal:  J Virol       Date:  2016-01-06       Impact factor: 5.103
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