Literature DB >> 23303500

A real-time PCR assay for rapid detection and quantification of Exserohilum rostratum, a causative pathogen of fungal meningitis associated with injection of contaminated methylprednisolone.

Yanan Zhao1, Ruta Petraitiene, Thomas J Walsh, David S Perlin.   

Abstract

A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg.

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Year:  2013        PMID： 23303500      PMCID： PMC3592047          DOI： 10.1128/JCM.03369-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens.

Authors:  Anna Lau; Sharon Chen; Tania Sorrell; Dee Carter; Richard Malik; Patricia Martin; Catriona Halliday
Journal:  J Clin Microbiol       Date:  2006-11-22       Impact factor: 5.948

2.  Fungal meningitis from injection of contaminated steroids: a compounding problem.

Authors:  Lucy E Wilson; David Blythe; Joshua M Sharfstein
Journal:  JAMA       Date:  2012-12-19       Impact factor: 56.272

Review 3.  Fungal infections associated with contaminated methylprednisolone injections.

Authors:  Carol A Kauffman; Peter G Pappas; Thomas F Patterson
Journal:  N Engl J Med       Date:  2012-10-19       Impact factor: 91.245

4.  Molecular identification and in vitro response to antifungal drugs of clinical isolates of Exserohilum.

Authors:  Keith Cássia da Cunha; Deanna A Sutton; Josepa Gené; Javier Capilla; Josep Cano; Josep Guarro
Journal:  Antimicrob Agents Chemother       Date:  2012-06-25       Impact factor: 5.191

5.  Detection of Aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real-time nucleic acid sequence-based amplification.

Authors:  Yanan Zhao; Steven Park; Peter Warn; Raghdaa Shrief; Elizabeth Harrison; David S Perlin
Journal:  J Clin Microbiol       Date:  2010-02-03       Impact factor: 5.948

Review 6.  PCR-based diagnosis of human fungal infections.

Authors:  Prasanna D Khot; David N Fredricks
Journal:  Expert Rev Anti Infect Ther       Date:  2009-12       Impact factor: 5.091

7.  Multistate outbreak of fungal infection associated with injection of methylprednisolone acetate solution from a single compounding pharmacy - United States, 2012.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2012-10-19       Impact factor: 17.586

8.  High-frequency triazole resistance found In nonculturable Aspergillus fumigatus from lungs of patients with chronic fungal disease.

Authors:  David W Denning; Steven Park; Cornelia Lass-Florl; Marcin G Fraczek; Marie Kirwan; Robin Gore; Jaclyn Smith; Ahmed Bueid; Caroline B Moore; Paul Bowyer; David S Perlin
Journal:  Clin Infect Dis       Date:  2011-05       Impact factor: 9.079

9.  Compounding drugs contaminated with fungi: a recipe for disaster.

Authors:  Stuart M Levitz
Journal:  Emerg Microbes Infect       Date:  2012-11       Impact factor: 7.163
  9 in total
  11 in total

1.  Fungal disease following contaminated steroid injections: Exserohilum is ready for its close-up.

Authors:  Marta Feldmesser
Journal:  Am J Pathol       Date:  2013-07-19       Impact factor: 4.307

2.  What can we learn and what do we need to know amidst the iatrogenic outbreak of Exserohilum rostratum meningitis?

Authors:  Dimitrios P Kontoyiannis; David S Perlin; Emmanuel Roilides; Thomas J Walsh
Journal:  Clin Infect Dis       Date:  2013-05-06       Impact factor: 9.079

3.  Not over yet: fungal infections following methyl prednisolone injections smoulder on.

Authors:  Rosemary Barnes; P Lewis White
Journal:  J Clin Microbiol       Date:  2014-09       Impact factor: 5.948

4.  Fungal DNA detected in blood samples of patients who received contaminated methylprednisolone injections reveals increased complexity of causative agents.

Authors:  Yanan Zhao; Emilian Armeanu; Richard DiVerniero; Terri A Lewis; Richard C Dobson; Dimitrios P Kontoyiannis; Emmanuel Roilides; Thomas J Walsh; David S Perlin
Journal:  J Clin Microbiol       Date:  2014-04-09       Impact factor: 5.948

Review 5.  Black Molds and Melanized Yeasts Pathogenic to Humans.

Authors:  Anuradha Chowdhary; John Perfect; G Sybren de Hoog
Journal:  Cold Spring Harb Perspect Med       Date:  2014-11-10       Impact factor: 6.915

6.  Utility of real-time PCR for detection of Exserohilum rostratum in body and tissue fluids during the multistate outbreak of fungal meningitis and other infections.

Authors:  Lalitha Gade; Dale E Grgurich; Thomas M Kerkering; Mary E Brandt; Anastasia P Litvintseva
Journal:  J Clin Microbiol       Date:  2014-12-17       Impact factor: 5.948

Review 7.  Iatrogenic fungal infections of central nervous system.

Authors:  Sourabh Lahoti; Joseph R Berger
Journal:  Curr Neurol Neurosci Rep       Date:  2013-11       Impact factor: 5.081

8.  Detection of fungal DNA in human body fluids and tissues during a multistate outbreak of fungal meningitis and other infections.

Authors:  Lalitha Gade; Christina M Scheel; Cau D Pham; Mark D Lindsley; Naureen Iqbal; Angela Ahlquist Cleveland; Anne M Whitney; Shawn R Lockhart; Mary E Brandt; Anastasia P Litvintseva
Journal:  Eukaryot Cell       Date:  2013-03-01

Review 9.  Mold infections of the central nervous system.

Authors:  Matthew McCarthy; Axel Rosengart; Audrey N Schuetz; Dimitrios P Kontoyiannis; Thomas J Walsh
Journal:  N Engl J Med       Date:  2014-07-10       Impact factor: 91.245

10.  Preliminary laboratory report of fungal infections associated with contaminated methylprednisolone injections.

Authors:  Shawn R Lockhart; Cau D Pham; Lalitha Gade; Naureen Iqbal; Christina M Scheel; Angela A Cleveland; Anne M Whitney; Judith Noble-Wang; Tom M Chiller; Benjamin J Park; Anastasia P Litvintseva; Mary E Brandt
Journal:  J Clin Microbiol       Date:  2013-06-12       Impact factor: 5.948
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