BACKGROUND: The multi-drug resistant Shigella has posed a therapeutic challenge in most parts of the world. In the last few years, there has been a tremendous change in the anti-microbial susceptibility profile of this organism. AIM: This present study was carried out to determine the current anti-microbial susceptibility pattern of the members of the genus, Shigella in our region. MATERIALS AND METHODS: Two thausand six hundred fifty eight stool samples from patients with diarrhoea were received between 2008 and 2010. The disc diffusion testing was performed by the Kirby-Bauer method and the minimum inhibitory concentrations (MICs) of ciprofloxacin and ceftriaxone were obtained by the agar dilution method and the E-test. The double disk synergy test was used to confirm the status of the extended beta-lactamase producers. RESULTS: 74 (2.78%) Shigella spp were isolated, out of which S.flexneri was 90.54%, S.dysenteriae was 2.70%, S.boydii was 1.35% and S.sonnei was 5.40%. 43 (58.108%) strains were isolated from children of 0 to ≤5 years, 13(17.56%) were isolated from children who were >5 years but ≤ 15 years of age and the rest of the 18 (24.32%) were isolated from adult patients. 79% of the strains were resistant to ampicillin, followed by 51% which were resistant to nalidixic acid, followed by 50% which were resistant to ciprofloxacin (the MIC of ciprofloxacin was 16μg/ml), and 39.4% which were resistant to furoxone and chloramphenicol respectively. 2 (3%) strains of S.flexneri were found to be resistant to ceftriaxone, which had MICs of > 256µg/ml. The ceftriaxone resistant S. flexneri isolates were confirmed to be extended spectrum beta-lactamase producers by the double disk synergy test. CONCLUSION: The continuous assessment of the anti-microbial susceptibility patterns and the periodic reporting in this context is important.
BACKGROUND: The multi-drug resistant Shigella has posed a therapeutic challenge in most parts of the world. In the last few years, there has been a tremendous change in the anti-microbial susceptibility profile of this organism. AIM: This present study was carried out to determine the current anti-microbial susceptibility pattern of the members of the genus, Shigella in our region. MATERIALS AND METHODS: Two thausand six hundred fifty eight stool samples from patients with diarrhoea were received between 2008 and 2010. The disc diffusion testing was performed by the Kirby-Bauer method and the minimum inhibitory concentrations (MICs) of ciprofloxacin and ceftriaxone were obtained by the agar dilution method and the E-test. The double disk synergy test was used to confirm the status of the extended beta-lactamase producers. RESULTS: 74 (2.78%) Shigella spp were isolated, out of which S.flexneri was 90.54%, S.dysenteriae was 2.70%, S.boydii was 1.35% and S.sonnei was 5.40%. 43 (58.108%) strains were isolated from children of 0 to ≤5 years, 13(17.56%) were isolated from children who were >5 years but ≤ 15 years of age and the rest of the 18 (24.32%) were isolated from adult patients. 79% of the strains were resistant to ampicillin, followed by 51% which were resistant to nalidixic acid, followed by 50% which were resistant to ciprofloxacin (the MIC of ciprofloxacin was 16μg/ml), and 39.4% which were resistant to furoxone and chloramphenicol respectively. 2 (3%) strains of S.flexneri were found to be resistant to ceftriaxone, which had MICs of > 256µg/ml. The ceftriaxone resistant S. flexneri isolates were confirmed to be extended spectrum beta-lactamase producers by the double disk synergy test. CONCLUSION: The continuous assessment of the anti-microbial susceptibility patterns and the periodic reporting in this context is important.
Authors: Ha Vinh; Stephen Baker; James Campbell; Nguyen Van Minh Hoang; Ha Thi Loan; Mai Thu Chinh; Vo Thi Cuc Anh; To Song Diep; Le Thi Phuong; Constance Schultsz; Jeremy Farrar Journal: J Med Microbiol Date: 2009-02 Impact factor: 2.472
Authors: Lorenz von Seidlein; Deok Ryun Kim; Mohammad Ali; Hyejon Lee; Xuanyi Wang; Vu Dinh Thiem; Do Gia Canh; Wanpen Chaicumpa; Magdarina D Agtini; Anowar Hossain; Zulfiqar A Bhutta; Carl Mason; Ornthipa Sethabutr; Kaisar Talukder; G B Nair; Jacqueline L Deen; Karen Kotloff; John Clemens Journal: PLoS Med Date: 2006-09 Impact factor: 11.069
Authors: Matthew A Croxen; Robyn J Law; Roland Scholz; Kristie M Keeney; Marta Wlodarska; B Brett Finlay Journal: Clin Microbiol Rev Date: 2013-10 Impact factor: 26.132