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Literature DB >> 2326165

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.

Abstract

Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.

Entities: Chemical

Mesh：

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Year: 1990 PMID： 2326165 PMCID： PMC330274 DOI： 10.1093/nar/18.2.351

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

7 in total

1. Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors: M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal: Cell Date: 1979-04 Impact factor: 41.582

2. A general method for isolation of high molecular weight DNA from eukaryotes.

Authors: N Blin; D W Stafford
Journal: Nucleic Acids Res Date: 1976-09 Impact factor: 16.971

3. DNA methylation prevents transfection of genes for specific surface antigens.

Authors: S Alberti; L A Herzenberg
Journal: Proc Natl Acad Sci U S A Date: 1988-11 Impact factor: 11.205

4. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

Authors: A K Sood; D Pereira; S M Weissman
Journal: Proc Natl Acad Sci U S A Date: 1981-01 Impact factor: 11.205

5. The integrated genome of murine leukemia virus.

Authors: D Steffen; R A Weinberg
Journal: Cell Date: 1978-11 Impact factor: 41.582

6. Quantitative karyotyping of human chromosomes by dual beam flow cytometry.

Authors: R G Langlois; L C Yu; J W Gray; A V Carrano
Journal: Proc Natl Acad Sci U S A Date: 1982-12 Impact factor: 11.205

7. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors: J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal: Biochemistry Date: 1979-11-27 Impact factor: 3.162

7 in total

1. Molecular cloning, reconstruction and expression of the gene encoding the alpha-chain of the bovine CD8--definition of three peptide regions conserved across species.

Authors: P Lalor; C Bucci; M Fornaro; M C Rattazzi; H Nakauchi; L A Herzenberg; S Alberti
Journal: Immunology Date: 1992-05 Impact factor: 7.397

Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.

1. Transformation of mammalian cells with genes from procaryotes and eucaryotes.

2. A general method for isolation of high molecular weight DNA from eukaryotes.

3. DNA methylation prevents transfection of genes for specific surface antigens.

4. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.

5. The integrated genome of murine leukemia virus.

6. Quantitative karyotyping of human chromosomes by dual beam flow cytometry.

7. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

1. Molecular cloning, reconstruction and expression of the gene encoding the alpha-chain of the bovine CD8--definition of three peptide regions conserved across species.

2. Development of a direct DNA extraction protocol for real-time PCR detection of Giardia lamblia from surface water.

3. A novel nucleus-targeted protein is expressed in barley leaves during senescence and pathogen infection.

4. Long-range transcriptome sequencing reveals cancer cell growth regulatory chimeric mRNA.

5. Polybrene/DMSO-assisted gene transfer. Generating stable transfectants with nanogram amounts of DNA.

6. DNA methylation prevents the amplification of TROP1, a tumor-associated cell surface antigen gene.

7. A seven-gene CpG-island methylation panel predicts breast cancer progression.