| Literature DB >> 23239458 |
Elke Socher1, Barbara Imperiali.
Abstract
Caught in the act: The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals.Entities:
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Year: 2012 PMID: 23239458 PMCID: PMC3776414 DOI: 10.1002/cbic.201200700
Source DB: PubMed Journal: Chembiochem ISSN: 1439-4227 Impact factor: 3.164