Literature DB >> 22764096

Converting a solvatochromic fluorophore into a protein-based pH indicator for extreme acidity.

Maiyun Yang1, Yanqun Song, Meng Zhang, Shixian Lin, Ziyang Hao, Yuan Liang, Dianmu Zhang, Peng R Chen.   

Abstract

Live-cell pH measurements: An environment-sensitive fluorophore (green) was site-specifically introduced on HdeA, an acid-resistant chaperone showing pH-mediated conformational changes under low pH conditions. A survey of the attachment sites led to the discovery of one position on HdeA at which the attached fluorophore showed a strong fluorescence increase upon acidification.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 22764096     DOI： 10.1002/anie.201204029

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  12 in total

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6.  FRET-capture: a sensitive method for the detection of dynamic protein interactions.

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7.  Discovery of allosteric modulators for GABAA receptors by ligand-directed chemistry.

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Journal:  Nat Chem Biol       Date:  2016-08-15       Impact factor: 15.040

8.  Solvatoemissive dual fluorescence of N-(pyridylmethyl)-3-nitro-1,8-naphthalimides.

Authors:  Jayanta K Nath; Jubaraj B Baruah
Journal:  J Fluoresc       Date:  2014-02-11       Impact factor: 2.217

9.  Biocompatible click chemistry enabled compartment-specific pH measurement inside E. coli.

Authors:  Maiyun Yang; Abubakar S Jalloh; Wei Wei; Jing Zhao; Peng Wu; Peng R Chen
Journal:  Nat Commun       Date:  2014-09-19       Impact factor: 14.919

10.  Copper-Catalyzed Click Reaction on/in Live Cells.

Authors:  Siheng Li; Lin Wang; Fei Yu; Zhiling Zhu; Dema Shobaki; Haoqing Chen; Mu Wang; Jun Wang; Guoting Qin; Uriel J Erasquin; Li Ren; Yingjun Wang; Chengzhi Cai
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