| Literature DB >> 23231891 |
V M Corman1, M A Müller, U Costabel, J Timm, T Binger, B Meyer, P Kreher, E Lattwein, M Eschbach-Bludau, A Nitsche, T Bleicker, O Landt, B Schweiger, J F Drexler, A D Osterhaus, B L Haagmans, U Dittmer, F Bonin, T Wolff, C Drosten.
Abstract
We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.Entities:
Mesh：
Substances：
Year: 2012 PMID： 23231891 DOI： 10.2807/ese.17.49.20334-en
Source DB: PubMed Journal: Euro Surveill ISSN： 1025-496X