PURPOSE: Arterial wall mass transport properties dictate local distribution of biomolecules or locally delivered dugs. Knowing how these properties vary between coronary artery locations could provide insight into how therapy efficacy is altered between arterial locations. METHODS: We introduced an indocarbocyanine drug surrogate to the lumens of left anterior descending and right coronary (LADC; RC) arteries from pigs with or without a pressure gradient. Interstitial fluorescent intensity was measured on live samples with multiphoton microscopy. We also measured binding to porcine coronary SMCs in monoculture. RESULTS: Diffusive transport constants peaked in the middle sections of the LADC and RC arteries by 2.09 and 2.04 times, respectively, compared to the proximal and distal segments. There was no statistical difference between the average diffusivity value between LADC and RC arteries. The convection coefficients had an upward trend down each artery, with the RC being higher than the LADC by 3.89 times. CONCLUSIONS: This study demonstrates that the convective and diffusive transport of lipophilic molecules changes between the LADC and the RC arteries as well as along their length. These results may have important implications in optimizing drug delivery for the treatment of coronary artery disease.
PURPOSE: Arterial wall mass transport properties dictate local distribution of biomolecules or locally delivered dugs. Knowing how these properties vary between coronary artery locations could provide insight into how therapy efficacy is altered between arterial locations. METHODS: We introduced an indocarbocyanine drug surrogate to the lumens of left anterior descending and right coronary (LADC; RC) arteries from pigs with or without a pressure gradient. Interstitial fluorescent intensity was measured on live samples with multiphoton microscopy. We also measured binding to porcine coronary SMCs in monoculture. RESULTS: Diffusive transport constants peaked in the middle sections of the LADC and RC arteries by 2.09 and 2.04 times, respectively, compared to the proximal and distal segments. There was no statistical difference between the average diffusivity value between LADC and RC arteries. The convection coefficients had an upward trend down each artery, with the RC being higher than the LADC by 3.89 times. CONCLUSIONS: This study demonstrates that the convective and diffusive transport of lipophilic molecules changes between the LADC and the RC arteries as well as along their length. These results may have important implications in optimizing drug delivery for the treatment of coronary artery disease.
Authors: Dale T Ashby; George Dangas; Roxana Mehran; Alexandra J Lansky; Raj Narasimaiah; Ioannis Iakovou; Sotir Polena; Lowell F Satler; Augusto D Pichard; Kenneth M Kent; Gregg W Stone; Martin B Leon Journal: Am J Cardiol Date: 2002-05-15 Impact factor: 2.778
Authors: Joseph T Keyes; Stacy M Borowicz; Jacob H Rader; Urs Utzinger; Mohamad Azhar; Jonathan P Vande Geest Journal: Microsc Microanal Date: 2011-04 Impact factor: 4.127
Authors: Giju Thomas; Johan van Voskuilen; Hoa Truong; Ji-Ying Song; Hans C Gerritsen; H J C M Sterenborg Journal: Biomed Opt Express Date: 2014-11-13 Impact factor: 3.732
Authors: Michelle Hine Armstrong; Adrián Buganza Tepole; Ellen Kuhl; Bruce R Simon; Jonathan P Vande Geest Journal: PLoS One Date: 2016-04-14 Impact factor: 3.240