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Literature DB >> 23201267

A malachite green-based assay to assess glucan phosphatase activity.

Amanda R Sherwood¹, Bradley C Paasch, Carolyn A Worby, Matthew S Gentry.

Abstract

With the recent discovery of a unique class of dual-specificity phosphatases that dephosphorylate glucans, we report an in vitro assay tailored for the detection of phosphatase activity against phosphorylated glucans. We demonstrate that, in contrast to a general phosphatase assay using a synthetic substrate, only phosphatases that possess glucan phosphatase activity liberate phosphate from the phosphorylated glucan amylopectin using the described assay. This assay is simple and cost-effective, providing reproducible results that clearly establish the presence or absence of glucan phosphatase activity. The assay described will be a useful tool in characterizing emerging members of the glucan phosphatase family.

Entities: Chemical Disease Gene Mutation Species

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Year: 2012 PMID： 23201267 PMCID： PMC3577954 DOI： 10.1016/j.ab.2012.10.044

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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