Literature DB >> 23196788

Is blood agar an alternative to sabouraud dextrose agar for the isolation of fungi in patients with mycotic keratitis.

Ashok Kumar Reddy1, Upputuri Brahmaiah, Nitesh Narayen, Ravi Kumar Reddy, Rupak Kumar Reddy, Meghraj Chitta, Srinivas Prasad, Rishi Swarup, Syed Maaz Mohiuddin, Madhukar Reddy, Murali K Aasuri, B S R Murthy, Milind Bhide, Sajid Ahmed.   

Abstract

To compare the blood agar (BA), sabouraud dextrose agar (SDA) and chocolate agar (CA) for the isolation of fungi in patients with mycotic keratitis. Corneal Scrapings of 229 patients with clinically diagnosed microbial keratitis were inoculated on BA, SDA, CA. The culture media were evaluated for the rate and time taken for the fungal growth. Seventy six of 229 patients had fungal keratitis. Fungus grew on BA in 60/76(78.9 %), on SDA in 76/76 (100 %), on CA in 40/76(52.6 %) patients. The fungi which grew on BA (60/76) also grown on SDA at the same time. The colony morphologies of different fungi were better on SDA than BA/CA. Among the different culture media, SDA is essential for the isolation fungi in patients with mycotic keratitis.

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Year:  2012        PMID: 23196788     DOI: 10.1007/s10792-012-9683-5

Source DB:  PubMed          Journal:  Int Ophthalmol        ISSN: 0165-5701            Impact factor:   2.031


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