Literature DB >> 2315314

Recombinant human bone morphogenetic protein induces bone formation.

E A Wang1, V Rosen, J S D'Alessandro, M Bauduy, P Cordes, T Harada, D I Israel, R M Hewick, K M Kerns, P LaPan.   

Abstract

We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2315314      PMCID： PMC53658          DOI： 10.1073/pnas.87.6.2220

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Solubilized and insolubilized bone morphogenetic protein.

Authors:  M R Urist; A Mikulski; A Lietze
Journal:  Proc Natl Acad Sci U S A       Date:  1979-04       Impact factor: 11.205

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Synthetic peptides as antigens: pitfalls of conjugation methods.

Authors:  J P Briand; S Muller; M H Van Regenmortel
Journal:  J Immunol Methods       Date:  1985-04-08       Impact factor: 2.303

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

7.  Bone cell differentiation and growth factors.

Authors:  M R Urist; R J DeLange; G A Finerman
Journal:  Science       Date:  1983-05-13       Impact factor: 47.728

8.  Homology of bone-inductive proteins from human, monkey, bovine, and rat extracellular matrix.

Authors:  T K Sampath; A H Reddi
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

9.  Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography.

Authors:  M R Urist; Y K Huo; A G Brownell; W M Hohl; J Buyske; A Lietze; P Tempst; M Hunkapiller; R J DeLange
Journal:  Proc Natl Acad Sci U S A       Date:  1984-01       Impact factor: 11.205

10.  Novel regulators of bone formation: molecular clones and activities.

Authors:  J M Wozney; V Rosen; A J Celeste; L M Mitsock; M J Whitters; R W Kriz; R M Hewick; E A Wang
Journal:  Science       Date:  1988-12-16       Impact factor: 47.728
View more
  198 in total

Review 1.  Growth regulatory factors and bone.

Authors:  G R Mundy; D Chen; M Zhao; S Dallas; C Xu; S Harris
Journal:  Rev Endocr Metab Disord       Date:  2001-01       Impact factor: 6.514

2.  Parathyroid hormone treatment improves the cortical bone microstructure by improving the distribution of type I collagen in postmenopausal women with osteoporosis.

Authors:  Maria-Grazia Ascenzi; Vivian P Liao; Brittany M Lee; Fabrizio Billi; Hua Zhou; Robert Lindsay; Felicia Cosman; Jeri Nieves; John P Bilezikian; David W Dempster
Journal:  J Bone Miner Res       Date:  2012-03       Impact factor: 6.741

3.  Stat1 functions as a cytoplasmic attenuator of Runx2 in the transcriptional program of osteoblast differentiation.

Authors:  Sunhwa Kim; Takako Koga; Miho Isobe; Britt E Kern; Taeko Yokochi; Y Eugene Chin; Gerard Karsenty; Tadatsugu Taniguchi; Hiroshi Takayanagi
Journal:  Genes Dev       Date:  2003-08-15       Impact factor: 11.361

4.  Use of an osteoinductive biomaterial as a bone morphogenetic protein carrier.

Authors:  H Yuan; J D De Bruijn; X Zhang; C A Van Blitterswijk; K De Groot
Journal:  J Mater Sci Mater Med       Date:  2001-09       Impact factor: 3.896

5.  [Evaluation of the osteoinductive potential of genetically modified BMP-2 variants].

Authors:  K K Würzler; J Emmert; F Eichelsbacher; N R Kübler; W Sebald; J F Reuther
Journal:  Mund Kiefer Gesichtschir       Date:  2004-02-03

6.  [Applying an osteoinductive protein complex for regeneration of osseous defects].

Authors:  F R Kloss; K A Schlegel; E Felszeghy; S Falk; J Wiltfang
Journal:  Mund Kiefer Gesichtschir       Date:  2004-01-20

7.  Optimally porous and biomechanically compatible scaffolds for large-area bone regeneration.

Authors:  Ami R Amini; Douglas J Adams; Cato T Laurencin; Syam P Nukavarapu
Journal:  Tissue Eng Part A       Date:  2012-04-16       Impact factor: 3.845

8.  Injectable thermogelling chitosan for the local delivery of bone morphogenetic protein.

Authors:  Shaun W McLaughlin; Zhanwu Cui; Trevor Starnes; Cato T Laurencin; Ho-Man Kan; Qian Wu; Lakshmi S Nair
Journal:  J Mater Sci Mater Med       Date:  2012-06-03       Impact factor: 3.896

9.  Identification of transforming growth factor beta family members present in bone-inductive protein purified from bovine bone.

Authors:  A J Celeste; J A Iannazzi; R C Taylor; R M Hewick; V Rosen; E A Wang; J M Wozney
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

10.  The balance of WNT and FGF signaling influences mesenchymal stem cell fate during skeletal development.

Authors:  Takamitsu Maruyama; Anthony J Mirando; Chu-Xia Deng; Wei Hsu
Journal:  Sci Signal       Date:  2010-05-25       Impact factor: 8.192
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.