Literature DB >> 23129116

Approaches to studying Arf GAPs in cells: in vitro assay with isolated focal adhesions.

Pei-Wen Chen1, Xiaoying Jian1, Ruibai Luo1, Paul A Randazzo1.   

Abstract

The Arf GAPs are a family of proteins with a common catalytic function of hydrolyzing GTP bound to ADP-ribosylation factors (Arf) with proposed cellular functions that are diverse (Inoue and Randazzo, 2007; Kahn et al., 2008). Understanding the biochemistry of the Arf GAPs is valuable for designing and interpreting experiments using standard cell biology techniques described elsewhere. The following briefly reviews some common approaches for in vivo studies of Arf GAPs and discusses the use of isolated cellular organelles to complement in vivo experiments. Detailed protocols for examining the activity of Arf GAPs in whole cell lysates and in association with isolated focal adhesions are provided.

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Year:  2012        PMID： 23129116      PMCID： PMC9454313          DOI： 10.1002/0471143030.cb1713s55

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  35 in total

1.  Assay and purification of phosphoinositide-dependent ADP-ribosylation factor (ARF) GTPase activating proteins.

Authors:  P A Randazzo; K Miura; T R Jackson
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

2.  Biochemical characterization of the cell-biomaterial interface by quantitative proteomics.

Authors:  W Y Tong; Y M Liang; V Tam; H K Yip; Y T Kao; K M C Cheung; K W K Yeung; Y W Lam
Journal:  Mol Cell Proteomics       Date:  2010-06-20       Impact factor: 5.911

3.  A rapid, sensitive, and specific method for the determination of protein in dilute solution.

Authors:  W Schaffner; C Weissmann
Journal:  Anal Biochem       Date:  1973-12       Impact factor: 3.365

4.  Kinetic dependence of phospholipase A 2 activity on the detergent Triton X-100.

Authors:  E A Dennis
Journal:  J Lipid Res       Date:  1973-03       Impact factor: 5.922

5.  Phosphatidylinositol-4-phosphate 5-kinase and GEP100/Brag2 protein mediate antiangiogenic signaling by semaphorin 3E-plexin-D1 through Arf6 protein.

Authors:  Atsuko Sakurai; Xiaoying Jian; Charity J Lee; Yosif Manavski; Emmanouil Chavakis; Julie Donaldson; Paul A Randazzo; J Silvio Gutkind
Journal:  J Biol Chem       Date:  2011-07-27       Impact factor: 5.157

Review 6.  The small G proteins of the Arf family and their regulators.

Authors:  Alison K Gillingham; Sean Munro
Journal:  Annu Rev Cell Dev Biol       Date:  2007       Impact factor: 13.827

Review 7.  ARF family G proteins and their regulators: roles in membrane transport, development and disease.

Authors:  Julie G Donaldson; Catherine L Jackson
Journal:  Nat Rev Mol Cell Biol       Date:  2011-05-18       Impact factor: 94.444

8.  Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.

Authors:  Jean-Cheng Kuo; Xuemei Han; Cheng-Te Hsiao; John R Yates; Clare M Waterman
Journal:  Nat Cell Biol       Date:  2011-03-20       Impact factor: 28.824

Review 9.  Nomenclature for the human Arf family of GTP-binding proteins: ARF, ARL, and SAR proteins.

Authors:  Richard A Kahn; Jacqueline Cherfils; Marek Elias; Ruth C Lovering; Sean Munro; Annette Schurmann
Journal:  J Cell Biol       Date:  2006-02-27       Impact factor: 10.539

10.  Two human ARFGAPs associated with COP-I-coated vesicles.

Authors:  Gabriella Frigerio; Neil Grimsey; Martin Dale; Irina Majoul; Rainer Duden
Journal:  Traffic       Date:  2007-09-29       Impact factor: 6.215
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  8 in total

1.  ARAP2 signals through Arf6 and Rac1 to control focal adhesion morphology.

Authors:  Pei-Wen Chen; Xiaoying Jian; Hye-Young Yoon; Paul A Randazzo
Journal:  J Biol Chem       Date:  2013-01-07       Impact factor: 5.157

2.  The BAR domain of the Arf GTPase-activating protein ASAP1 directly binds actin filaments.

Authors:  Pei-Wen Chen; Neil Billington; Ben Y Maron; Jeffrey A Sload; Krishna Chinthalapudi; Sarah M Heissler
Journal:  J Biol Chem       Date:  2020-05-22       Impact factor: 5.157

3.  Interaction of the N terminus of ADP-ribosylation factor with the PH domain of the GTPase-activating protein ASAP1 requires phosphatidylinositol 4,5-bisphosphate.

Authors:  Neeladri Sekhar Roy; Xiaoying Jian; Olivier Soubias; Peng Zhai; Jessica R Hall; Jessica N Dagher; Nathan P Coussens; Lisa M Jenkins; Ruibai Luo; Itoro O Akpan; Matthew D Hall; R Andrew Byrd; Marielle E Yohe; Paul A Randazzo
Journal:  J Biol Chem       Date:  2019-10-06       Impact factor: 5.157

4.  The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.

Authors:  Pei-Wen Chen; Xiaoying Jian; Sarah M Heissler; Kang Le; Ruibai Luo; Lisa M Jenkins; Attila Nagy; Joel Moss; James R Sellers; Paul A Randazzo
Journal:  J Biol Chem       Date:  2016-02-17       Impact factor: 5.157

5.  Molecular Basis for Cooperative Binding of Anionic Phospholipids to the PH Domain of the Arf GAP ASAP1.

Authors:  Xiaoying Jian; Wai-Kwan Tang; Peng Zhai; Neeladri Sekhar Roy; Ruibai Luo; James M Gruschus; Marielle E Yohe; Pei-Wen Chen; Yifei Li; R Andrew Byrd; Di Xia; Paul A Randazzo
Journal:  Structure       Date:  2015-09-10       Impact factor: 5.006

6.  Quantitative Analysis of Guanine Nucleotide Exchange Factors (GEFs) as Enzymes.

Authors:  Paul A Randazzo; Xiaoying Jian; Pei-Wen Chen; Peng Zhai; Olivier Soubias; John K Northup
Journal:  Cell Logist       Date:  2014-01-09

7.  Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2.

Authors:  Lei Yu; Si Gui; Yawei Liu; Xiaoyu Qiu; Guozhong Zhang; Xi'an Zhang; Jun Pan; Jun Fan; Songtao Qi; Binghui Qiu
Journal:  Aging (Albany NY)       Date:  2019-08-05       Impact factor: 5.682

Review 8.  Role of exosomes in malignant glioma: microRNAs and proteins in pathogenesis and diagnosis.

Authors:  Amir B Ghaemmaghami; Maryam Mahjoubin-Tehran; Ahmad Movahedpour; Korosh Morshedi; Amirhossein Sheida; Seyed Pouya Taghavi; Hamed Mirzaei; Michael R Hamblin
Journal:  Cell Commun Signal       Date:  2020-08-03       Impact factor: 7.525
  8 in total

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