The optimal screening interval for gastric cancer using esophago-gastro-duodenoscopy in Japan.

BACKGROUND: Gastric cancer is one of the most significant diseases, and esophago-gastro-duodenoscopy (EGD) is one of screening methods for gastric cancer. This study was conducted to identify the optimal screening interval for gastric cancer using EGD in healthy adults.
METHODS: A retrospective cohort study was conducted on 3,723 healthy participants without a known diagnosis of gastric cancer at baseline from January 2005 to December 2010. Participants underwent annual health screenings, including EGD, at the Center for Preventive Medicine at St Luke's International Hospital, a community teaching hospital in Japan. Participants with cytological abnormalities underwent further examination. A generalized estimating equation (GEE) was used to analyze the longitudinal data. We decided 0.5% of incidence of gastric cancer as a cutoff point for interval.
RESULTS: The mean age (SD) of the participants was 55 (11) years, and 1,879 (50.5%) were male. During the study period, gastric cancer was detected in 35 participants. However, the incidence varied based on their ages. In the age groups <40, 40-49, 50-59, 60-69 and ≥70 years old, the 5-year cumulative incidences (95%CI) of gastric cancer were 0% (0-0%), 0.3% (0.1-1.0%), 1.0% (0.5-1.8%), 1.4% (0.8-2.4%) and 1.9% (0.8-3.8%), respectively. The odds ratios of the incidence of gastric cancer per year, which were evaluated using GEE models for the age groups 40-49, 50-59, 60-69 and ≥70 years old, were 1.51 (95%CI: 0.91-2.49), 1.94 (95%CI: 1.31-2.86), 1.59 (95%CI: 1.23-2.06) and 1.46 (95%CI: 1.06-2.02), respectively.
CONCLUSIONS: A screening for gastric cancer using EGD may be appropriate annually for healthy people over 70 years old, every two or three years for people 60-69 years old and every four years for people 50-59 years old. People younger than 50 years old may only need repeat screenings every five years or more.

Background

Approximately 776,000 patients die from gastric cancer worldwide each year [1]. Japan has one of the highest rates of gastric cancer in the world; approximately 50,000 patients in Japan die from gastric cancer each year [2].

Some countries with high incidences of gastric cancer, including Japan [3], Chile [4] and Venezuela [5], recommend mass screening for gastric cancer, while other countries do not recommend routine screening [6]. In these high risk countries, mass screening for gastric cancer is expected to increase the early detection rate and reduce the mortality from gastric cancer based on previous large-scale case control studies and reviews [7,8].

Screening strategies for gastric cancer, however, are different among these high incidence countries. Esophago-gastro-duodenoscopy (EGD) is a screening method that is readily available and recommended by some experts in Japan [9]. Although there is a systematic review which recommends barium x-ray [10], the sensitivity of barium x-rays is lower than that of EGD. Considering the fact that approximately 40% of gastric cancers are still undetected [11], higher sensitivity of screening method is of paramount importance. Moreover, the incidence of complications during EGDs was reported as low as 0.012% and the mortality rate was only 0.001% in Japan [12]. Finally, EGD was shown to be cost effective in a systematic review [13].

However, the optimal interval of EGD screening for gastric cancer is yet to be determined. Therefore, we evaluated the optimal interval for EGD as a gastric cancer screening using a 5-year cohort.

Methods

Study participants

From January 2005 to December 2010, all participants (≥20 years old) who underwent EGD as a part of an annual health check-up program at the Center for Preventive Medicine at St. Luke’s International Hospital in Tokyo, Japan, were enrolled in this cohort study. We included all participants who have completed 5 years follow up. We also included patients who were found gastric cancer during study period, even if they have not completed the remaining years. We excluded people with a diagnosis of gastric cancer at baseline. Previous EGD was not restricted in our study.

Data collection

We extracted data from the electronic health records of participants who had annual health screenings in 2005 (baseline year), 2006 (year 1), 2007 (year 2), 2008 (year 3), 2009 (year 4) and 2010 (year 5). The participants diagnosed with gastric cancer based on cytology were further evaluated at the Department of Gastroenterological Medicine or Digestive Surgery at St. Luke’s International Hospital. The cytology was evaluated by two pathologists independently. We aggregated information from the participants who were determined to have gastric cancer by cytology and were evaluated at the other hospitals. The St. Luke’s International Hospital’s ethics committee approved the protocol for this study.

Measurements

The annual health check-up program contained an initial evaluation during which demographic and medical history information was collected. An EGD was performed by a gastroenterological specialist, and the cytological specimens were evaluated by two independent pathologists. Participants were divided into four age brackets: under 40 years of age, 40–49 years, 50–59 years, 60–69 years and 70 years or older. The incidence of gastric cancer, the odds ratio of the incidence over five years and the proportion of gastric cancers to the number of biopsies were used as indicators to define the optimal intervals for screening. An incidence of 0.5% for gastric cancer was considered the cutoff point based on a previous interval study and the Japanese prevalence of gastric cancer [14].

Statistical methods

Data were analyzed using descriptive statistics, including the mean, variance, standard deviation (SD), and percentage. Chi-squared and Fisher’s exact tests were used for cross-tabulated data, and t-tests for continuous data. Additionally, generalized estimating equations (GEE) [15], which were adjusted for possible risk factors, such as age, gender, alcohol consumption, smoking, family history, past medical history of gastric ulcers, salt intake and body mass index (BMI) [16-19], were used to evaluate the odds ratio of the cumulative incidence of gastric cancer [20]. GEE is performed to estimate the parameters of a generalized linear model with a possible unknown correlation between outcomes [21]. With this analysis, we can estimate likelihood with considering correlation matrix for the vector of repeated observations from each subject [22].

The majority of the data analyses were performed using the SPSS software 15.0J (SPSS Japan, Tokyo, Japan), except for the 95% confidence intervals (CI) based on an exact binominal [23] and the GEE methods which were calculated using the Stata version 10 (STATA Corp., College Station, TX, USA).

Results

From January 2005 to December 2010, 3,723 participants completed all five yearly health screenings. The mean age (SD) of participants was 55 (12) years, and 1,879 participants (50.5%) were male. The mean (SD) body mass index (BMI) was 22.5 (3.1) kg/m [2]. Of the participants, 685 (18.4) had a family history of gastric cancer, and 242 (6.5) had a past medical history of gastric cancer. Other baseline characteristics are shown in Table 1.

Table 1

Participant characteristics

	Gastric cancer patients over five years (n=35)	Normal participants (n=3,688)	Total (n=3,723)	Mean difference (p- value)
Age, mean (SD) years	62 (9)	55 (12)	55 (12)	<0.01
Gender, male (%) n	25 (71.4)	1,854 (50.3)	1,879 (50.5)	<0.01
BMI, mean (SD) kg/m [2]	23.8 (3.1)	22.5 (3.1)	22.5 (3.1)	0.01
SBP mean (SD), mmHg	125.4 (13.6)	120.7(18.2)	120.8 (18.2)	0.05
DBP mean (SD), mmHg	77.1 (7.9)	74.9 (11.3)	75.0 (11.3)	0.12
Heart rate mean (SD), bpm	69.5 (9.4)	73.4 (11.3)	73.3 (11.3)	0.04
White blood cell mean (SD), *10 [3] /μl	5.6 (1.5)	5.1 (1.4)	5.1 (1.4)	0.06
Hemoglobin mean (SD), g/dl	14.1 (1.0)	13.9 (1.3)	13.9 (1.3)	0.21
Platelet mean (SD), *10 [6] /μl	224.2 (43.5)	234.4 (51.0)	234.3 (51.0)	0.24
Fasting blood sugar mean (SD), mg/dl	108.3 (18.8)	101.5 (17.2)	101.6 (17.3)	0.39
HgbA1c mean (SD), %	5.3 (0.7)	5.2 (0.6)	5.2 (0.6)	0.27
LDL-cho mean (SD), mg/dl	117.0 (21.8)	120.5 (29.0)	120.5 (29.0)	0.47
HDL-cho mean (SD), mg/dl	58.2 (12.8)	62.8 (15.7)	62.7 (15.7)	0.08
TG mean (SD), mg/dl	107.4 (53.0)	102.8 (70.7)	102.8 (70.5)	0.70
Family history of gastric cancer n (%)	8 (1.2)	677 (18.4)	685 (18.4)	0.31
Alcohol habit n (%)	24 (68.6)	2,157 (58.5)	2,181 (58.6)	0.15
Smoker: Brinckman index n (SD)	430.7 (648.0)	210.6 (412.7)	21.7 (415.9)	0.05
Salt intake: g per day g (SD)	12.7 (3.4)	12.2 (3.8)	12.2 (3.8)	0.44
Gastric ulcer n (%)	3 (1.2)	239 (6.5)	242 (6.5)	0.40

Number of cigarettes smoked per day times the number of smoking years.

After 5 years, the cumulative incidence of gastric cancer was 0.9% (95% CI, 0.7-1.3). However, the incidence varied greatly between age groups. At 5 years, the participants who were <40, 40–49, 50–59, 60–69 and ≥70 years old at baseline had cumulative incidences (95% CI) of gastric cancer of 0% (0-0%), 0.3% (0.1-1.0%), 1.0% (0.5-1.8%), 1.4% (0.8-2.4%) and 1.9% (0.8-3.8%), respectively (Figure 1).

Figure 1

The cumulative incidence of gastric cancer for 5 years by age group.

During this 5 years period, 964 biopsies were performed, and 35 patients (3.6%, 95%CI: 2.5%-5.0%) were diagnosed with gastric cancer. All of them were followed throughout the study period (100%). Of these patients, 24 had adenocarcinoma, 6 had mucosa-associated lymphoid tissue (MALT) lymphoma, 4 had signet cell carcinoma, and 1 had carcinoid (Table 2). Among 24 patients with adenocarcinoma, 20 were able to be followed up with their stage of cancer. 17 patients were Stage IA, and 3 patients were Stage IB.

Table 2

Types of gastric cancer

	<40	40-49	50-59	60-69	≥70	Total
Adenocarcinoma	0	0	7	11	6	24
MALT*lymphoma	0	0	3	2	1	6
Signet cell carcinoma	0	3	1	0	0	4
Carcinoid	0	0	1	0	0	1
Total	0	3	12	13	7	35

MALT*: Mucosa-associated lymphoid tissue.

The patients with MALT lymphoma were treated by eradicating of H. pylori. Among other patients, 20 were determined to have cancer at an early stage and were treated with endoscopic submucosal dissection (ESD), and 9 underwent gastrectomy (Table 3).

Table 3

Types of treatment for gastric cancer

	<40	40-49	50-59	60-69	≥70	Total
ESD*	0	1	4	11	4	20
Gastrectomy	0	2	5	0	2	9
H. pylori eradication	0	0	3	2	1	6
Total	0	3	12	13	7	35

ESD*: Endoscopic submucosal dissection.

Table 4 shows the ratio of the incidence of gastric cancer per the number of biopsies. At 5 years, the participants who were <40, 40–49, 50–59, 60–69 and ≥70 years old at baseline had ratios (95% CI) of 0% (0-0%), 1.9% (0.4-5.4%), 3.5% (1.8-6.0%), 4.5% (2.4-7.6%) and 4.8% (2.0-9.7%), respectively. The ratio (95% CI) for all participants was 3.6 (2.5-5.0%).

Table 4

Positive biopsy ratio for gastric cancer over 5 years

	<40	40-49	50-59	60-69	≥70	Total
2006	0 / 4	0 / 27	0 / 55	2 / 57	2 / 18	4 / 161
2007	0 / 9	1 / 32	3 / 76	2 / 62	1 / 34	7 / 213
2008	0 / 4	1 / 35	1 / 70	2 / 53	2 / 26	6 / 188
2009	0 / 4	1 / 18	2 / 64	4 /67	2 / 35	9 / 188
2010	0 / 6	0 /47	6 / 79	3 /50	0 / 32	9 / 214
Total (%)	0 / 27 (0)	3 / 159 (1.9)	12 / 344 (3.5)	13 / 289 (4.5)	7 / 145 (4.8)	35 / 964 (3.6)

Incidence of gastric cancer / number of biopsies.

The odds ratios for the incidence of gastric cancer per year were estimated using GEE models adjusted for possible risk factors, including age, gender, alcohol consumption, smoking, family history, past medical history of gastric ulcers, salt intake and BMI. The independent odds ratios for the incidence of gastric cancer in the 40–49, 50–59, 60–69 and ≥70 years old age groups were 1.51 (95%CI: 0.91-2.49), 1.94 (95%CI: 1.31-2.86), 1.59 (95%CI: 1.23-2.06) and 1.46 (95%CI: 1.06-2.02), respectively. None of the participants who were <40 years old had gastric cancer. (Table 5) Although there was no statistical relationship between gastric cancer and other variables, high BMI tended to be associated with high incidence of gastric cancer.

Table 5

Odds ratios for the incidences of gastric cancer per year using a generalized estimating equation (GEE) in each age group, adjusted for age, gender, alcohol consumption, smoking, family history, past medical history of gastric ulcers, salt intake and body mass index (BMI)

Age	Odds Ratio	95% Confidence Interval	Standard Deviation	p-value
<40	No incidence
40-49	1.51	0.91 - 2.49	0.36	0.11
50-59	1.94	1.31 – 2.86	0.38	<0.01
60-69	1.59	1.23 – 2.06	0.21	<0.01
≥70	1.46	1.06 – 2.02	0.24	0.02

Discussion

This study showed that the incidence rates of gastric cancer significantly differed according to age. Accordingly, our recommended screening intervals were based on age brackets. For patients over 70 years old, screening for gastric cancer every year may be appropriate, and for patients 60–69 years old, every two or three years, for patients 50–59 years old, every three or four years, and for patients 40–49 years old and under 40 years old, every five years.

In the group of participants older than 70 years old, approximately 0.5% developed gastric cancer within the next year. The proportion of the incidence of gastric cancer to the number of biopsies was 7 / 145 (4.8%) compared to 35 / 964 (3.6%) in all participants. Having adjusted for multiple variables using GEE model, the odds ratio for the incidences of gastric cancer within the next year turned to be 1.46 (95%CI: 1.06-2.02). Cancer screening tends to be controversial in elderly people because of their remaining life expectancy. However, in Japan, the life expectancy is 80 years old for males and 86 years old for females [24], long enough for justifying cancer screening for people aged over 70 years.

For the 60–69 year-old age group, the incidence of gastric cancer exceeded 0.5% within the next three years. The proportion of the incidence of gastric cancer to the number of biopsies was 13 / 289 (4.5%), and the proportion was higher than for gastric cancer in all participants. In the GEE model, the odds ratio for the incidence of gastric cancer within the next year was 1.59 (95%CI: 1.23-2.06). Therefore, screening every two or three years may be appropriate.

For patients 50–59 years old, the incidence of gastric cancer was approximately 0.5% within the next four years. The proportion of the incidence of gastric cancer to the number of biopsies was 12 / 344 (3.5%), similar to the proportion of gastric cancer in all participants. Based on the GEE model, the odds ratio for the incidence of gastric cancer within the next year was 1.94 (95%CI: 1.31-2.86), and it was the highest among all the groups. Therefore, screening every four years may be reasonable.

For patients 40–49 years old, the incidence of gastric cancer was less than 0.5% within the next five years. The proportion of the incidence of gastric cancer to the number of biopsies was 3 / 159 (1.9%), and the proportion was lower than that for all participants. Based on the GEE model, the odds ratio for the incidence of gastric cancer in the next year was 1.51 (95%CI: 0.91-2.49), which attained no statistically significance. Therefore, screening every five or more years may be considered. However, in this group, all patients with gastric cancer had signet cell carcinoma, which has poor prognosis. This means that screening patients in this group may have to be considered carefully.

Among those under 40 years old, there were no patients with gastric cancer. Therefore, screening every five or more years may be appropriate for this age bracket.

In our study, 20 of 29 (69.0%, 95%CI: 49.2-84.7) patients with gastric cancer (excluding MALT lymphoma because of the differences in treatment) were detected in the early stage and treated with ESD. Compared to a previous study on barium x-ray screening program, the early stage detection rate in our study was high [25]. It is speculated that early detection of gastric cancer by nation which screening program in Japan has contributed to the reduced mortality from gastric cancer in the past decades [7]. In this regard, gastric cancer screening using EGD which detects gastric cancer in earlier stage than barium X-ray may be efficient.

In addition to that, early detection for gastric cancer was expected better prognosis than late detection. Previous study reported that the five years survival rate with gastric cancer in Stage I, II, III, IV were 99.1%, 72.6%, 45.9%, and 7.2% respectively in Japan [26]. Among 24 patients with adenocarcinoma, at least 20 patients were in Stage IA or IB in our study. Within them, 16 patients were treated by ESD, which was less invasive than gastrectomy. Therefore, patients in our study were expected not only early detection for gastric cancer, but also better prognosis.

There are some limitations to this study. First, this study was a retrospective cohort study. We could not determine the magnitude of false negative results. There is a report, however, which reported that the false negative rate for gastric cancer screening by EGD was nil while 12 months follow-up [27]. Therefore, we would think the effect of false negatives negligible in our study. Second, our data did not include information on H. pylori. It is said that 70-80% of people over 40 years old in Japan had H. pylori [[28]. A previous randomized controlled study showed that H. pylori eradication reduced precancerous lesions without affecting overall incidence of gastric cancer [29]. For this reason, the information on H. pylori infection is unlikely to affect our results from 5 years cohort study. Third, population in our study may have selection bias, because of the study design. However, the incidence of gastric cancer in our study was similar to that in the previous study in Japan [30]. Therefore, this is also unlikely to affect the result. Finally, our data didn’t have the information of atrophic gastritis. Previous study revealed that atrophic gastritis may be a major cause of gastric cancer [31]. For these reasons, additional studies are required to further evaluation.

Conclusions

A screening program for gastric cancer may be appropriate every year for healthy people over 70 years old, every two or three years for those 60–69 years old and every four years for those 50–59 years old. People younger than 50 years old may only need screenings every five years or more.

Competing interests

The authors declare that they have no competing pinterests.

Authors' contributions

DK conducted this study, decided study design, analyzed data, and drafted the manuscript. OT contributed to study design decision, performing data analysis and making manuscript. HA checked study design, reviewed manuscript and contribute to discussion. TF organized this study and contributed to discussion. All authors read and approved the final manuscript."

Pre-publication history

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http://www.biomedcentral.com/1471-230X/12/144/prepub