PURPOSE: Bladder urothelial carcinoma (bladder-UC) displays distinct genotypic differences compared to upper tract UC (UTUC). We recently reported specific 8q24 SNP variants confer susceptibility to UTUC and aggressive disease features. Herein, we evaluate a bladder-UC cohort to see whether similar polymorphisms are linked similarly same way with disease risk and aggressiveness. METHODS: 231 bladder-UC patients and 261 benign controls were matched for gender, age, ethnicity and smoking habits. We retrospectively retrieved information on tumour stage, grade, size, multiplicity, carcinoma in situ and tumour number. DNA was extracted from paraffin-embedded primary bladder-UC samples and blood of benign controls. Genotyping of rs9642880[T] (8q24.1) and rs798766[T] (4p16.3) was performed using commercially available Taqman(®) assays and the ABI™ 7000 Sequence Detector. RESULTS: Using a case-control analysis, bladder-UC risk was increased in individuals carrying the T/T genotype of rs9642880 [OR = 1.72 (95 % CI 1.1-2.8); p = 0.028] and rs798766 [OR = 1.84 (95 % CI 0.9-2.3); p = 0.01]. When analysing parameters of bladder-UC aggressiveness, the T/T genotypes for rs9642880 and rs798766 were not found to be associated with either grade [OR = 0.89 (95 % CI 0.52-1.32; p = 0.68) and OR = 0.95 (95 % CI 0.58-1.48; p = 0.61), respectively] or pathological stage [OR = 0.79 (95 % CI 0.42-1.48; p = 0.46) and OR = 0.90 (95 % CI 0.49-1.61; p = 0.72), respectively]. SNP variability of rs9642880[T] and rs798766[T] is associated with an increased risk of bladder-UC but we did not find an association with disease aggressiveness as we did previously for UTUC. CONCLUSIONS: This is further evidence of the distinct genetic differences that exist between bladder-UC and UTUC, and it is not possible to extrapolate results of genetic studies between these two urothelial disease entities.
PURPOSE:Bladder urothelial carcinoma (bladder-UC) displays distinct genotypic differences compared to upper tract UC (UTUC). We recently reported specific 8q24 SNP variants confer susceptibility to UTUC and aggressive disease features. Herein, we evaluate a bladder-UC cohort to see whether similar polymorphisms are linked similarly same way with disease risk and aggressiveness. METHODS: 231 bladder-UC patients and 261 benign controls were matched for gender, age, ethnicity and smoking habits. We retrospectively retrieved information on tumour stage, grade, size, multiplicity, carcinoma in situ and tumour number. DNA was extracted from paraffin-embedded primary bladder-UC samples and blood of benign controls. Genotyping of rs9642880[T] (8q24.1) and rs798766[T] (4p16.3) was performed using commercially available Taqman(®) assays and the ABI™ 7000 Sequence Detector. RESULTS: Using a case-control analysis, bladder-UC risk was increased in individuals carrying the T/T genotype of rs9642880 [OR = 1.72 (95 % CI 1.1-2.8); p = 0.028] and rs798766 [OR = 1.84 (95 % CI 0.9-2.3); p = 0.01]. When analysing parameters of bladder-UC aggressiveness, the T/T genotypes for rs9642880 and rs798766 were not found to be associated with either grade [OR = 0.89 (95 % CI 0.52-1.32; p = 0.68) and OR = 0.95 (95 % CI 0.58-1.48; p = 0.61), respectively] or pathological stage [OR = 0.79 (95 % CI 0.42-1.48; p = 0.46) and OR = 0.90 (95 % CI 0.49-1.61; p = 0.72), respectively]. SNP variability of rs9642880[T] and rs798766[T] is associated with an increased risk of bladder-UC but we did not find an association with disease aggressiveness as we did previously for UTUC. CONCLUSIONS: This is further evidence of the distinct genetic differences that exist between bladder-UC and UTUC, and it is not possible to extrapolate results of genetic studies between these two urothelial disease entities.
Authors: Lambertus A Kiemeney; Kjeld P van Houwelingen; Manon Bogaerts; J Alfred Witjes; Dorine W Swinkels; Martin den Heijer; Barbara Franke; Jack A Schalken; Gerald W Verhaegh Journal: Eur J Cancer Date: 2006-08-28 Impact factor: 9.162
Authors: Julius Gudmundsson; Patrick Sulem; Andrei Manolescu; Laufey T Amundadottir; Daniel Gudbjartsson; Agnar Helgason; Thorunn Rafnar; Jon T Bergthorsson; Bjarni A Agnarsson; Adam Baker; Asgeir Sigurdsson; Kristrun R Benediktsdottir; Margret Jakobsdottir; Jianfeng Xu; Thorarinn Blondal; Jelena Kostic; Jielin Sun; Shyamali Ghosh; Simon N Stacey; Magali Mouy; Jona Saemundsdottir; Valgerdur M Backman; Kristleifur Kristjansson; Alejandro Tres; Alan W Partin; Marjo T Albers-Akkers; Javier Godino-Ivan Marcos; Patrick C Walsh; Dorine W Swinkels; Sebastian Navarrete; Sarah D Isaacs; Katja K Aben; Theresa Graif; John Cashy; Manuel Ruiz-Echarri; Kathleen E Wiley; Brian K Suarez; J Alfred Witjes; Mike Frigge; Carole Ober; Eirikur Jonsson; Gudmundur V Einarsson; Jose I Mayordomo; Lambertus A Kiemeney; William B Isaacs; William J Catalona; Rosa B Barkardottir; Jeffrey R Gulcher; Unnur Thorsteinsdottir; Augustine Kong; Kari Stefansson Journal: Nat Genet Date: 2007-04-01 Impact factor: 38.330
Authors: Katja K H Aben; J Alfred Witjes; Mark P Schoenberg; Christina Hulsbergen-van de Kaa; André L M Verbeek; Lambertus A L M Kiemeney Journal: Int J Cancer Date: 2002-03-10 Impact factor: 7.396
Authors: Rayjean J Hung; Paolo Boffetta; Paul Brennan; Christian Malaveille; Agnès Hautefeuille; Francesco Donato; Umberto Gelatti; Massimiliano Spaliviero; Donatella Placidi; Angela Carta; Antonio Scotto di Carlo; Stefano Porru Journal: Int J Cancer Date: 2004-07-01 Impact factor: 7.396
Authors: James W F Catto; Abdel-Rahmene Azzouzi; Najla Amira; Ishtiaq Rehman; Kenneth M Feeley; Simon S Cross; Gaelle Fromont; Mathilde Sibony; Freddie C Hamdy; Oliver Cussenot; Mark Meuth Journal: Oncogene Date: 2003-11-27 Impact factor: 9.867
Authors: Jie Gao; Peng-Tao Yang; Yan Diao; Hua-Feng Kang; Yang Zhao; Shuai Lin; Zi-Ming Wang; Meng Wang; Xi-Jing Wang; Zhi-Jun Dai Journal: Int J Clin Exp Med Date: 2015-02-15
Authors: Il-Seok Lee; Sung Pil Seo; Yun Sok Ha; Pildu Jeong; Ho Won Kang; Won Tae Kim; Yong-June Kim; Seok Joong Yun; Sang Cheol Lee; Wun-Jae Kim Journal: J Biomed Res Date: 2017-01-19
Authors: Anne J Grotenhuis; Aleksandra M Dudek; Gerald W Verhaegh; J Alfred Witjes; Katja K Aben; Saskia L van der Marel; Sita H Vermeulen; Lambertus A Kiemeney Journal: PLoS One Date: 2014-02-25 Impact factor: 3.240