Literature DB >> 2302196

Substrate competition and specificity at the active site of amylopullulanase from Clostridium thermohydrosulfuricum.

S Mathupala1, B C Saha, J G Zeikus.   

Abstract

A highly thermostable pullulanase purified from Clostridium thermohydrosulfuricum strain 39E displayed dual activity with respect to glycosidic bond cleavage. The enzyme cleaved alpha-1,6 bonds in pullulan, while it showed alpha-1,4 activity against malto-oligosaccharides. Kinetic analysis of the purified enzyme in a system which contained both pullulan and amylose as the two competing substrates were used to distinguish the dual specificity of the enzyme from the single substrate specificity known for pullulanases and alpha-amylases.

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Year:  1990        PMID： 2302196     DOI： 10.1016/0006-291x(90)91920-n

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  13 in total

Review 1.  Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.

Authors:  C Vieille; G J Zeikus
Journal:  Microbiol Mol Biol Rev       Date:  2001-03       Impact factor: 11.056

2.  Ectopic expression of bacterial amylopullulanase enhances bioethanol production from maize grain.

Authors:  Hartinio N Nahampun; Chang Joo Lee; Jay-Lin Jane; Kan Wang
Journal:  Plant Cell Rep       Date:  2013-05-08       Impact factor: 4.570

3.  Characterization of Amylolytic Enzymes, Having Both alpha-1,4 and alpha-1,6 Hydrolytic Activity, from the Thermophilic Archaea Pyrococcus furiosus and Thermococcus litoralis.

Authors:  S H Brown; R M Kelly
Journal:  Appl Environ Microbiol       Date:  1993-08       Impact factor: 4.792

4.  Tracing the spread of fibronectin type III domains in bacterial glycohydrolases.

Authors:  E Little; P Bork; R F Doolittle
Journal:  J Mol Evol       Date:  1994-12       Impact factor: 2.395

Review 5.  Structure and function of α-glucan debranching enzymes.

Authors:  Marie Sofie Møller; Anette Henriksen; Birte Svensson
Journal:  Cell Mol Life Sci       Date:  2016-05-02       Impact factor: 9.261

Review 6.  Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity, or substrates.

Authors:  S E Lowe; M K Jain; J G Zeikus
Journal:  Microbiol Rev       Date:  1993-06

7.  Characterization of thermostable cyclodextrinase from Clostridium thermohydrosulfuricum 39E.

Authors:  B C Saha; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1990-09       Impact factor: 4.792

8.  Pullulanase of Thermoanaerobacterium thermosulfurigenes EM1 (Clostridium thermosulfurogenes): molecular analysis of the gene, composite structure of the enzyme, and a common model for its attachment to the cell surface.

Authors:  M Matuschek; G Burchhardt; K Sahm; H Bahl
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

9.  Novel maltotriose-hydrolyzing thermoacidophilic type III pullulan hydrolase from Thermococcus kodakarensis.

Authors:  Nasir Ahmad; Naeem Rashid; Muhammad Saleem Haider; Mehwish Akram; Muhammad Akhtar
Journal:  Appl Environ Microbiol       Date:  2013-12-02       Impact factor: 4.792

10.  Isolation and characterization of a heat-stable pullulanase from the hyperthermophilic archaeon Pyrococcus woesei after cloning and expression of its gene in Escherichia coli.

Authors:  A Rüdiger; P L Jorgensen; G Antranikian
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792
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