N M Ryabchenko1, O A Glavin, V V Shtefura, M F Anikushko. 1. RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine. nryabchenko@ukr.net
Abstract
AIM: Recent studies showed that increased chromosomal damage induced by ionizing radiation is observed among patients with different tumor types. The aim of the study was evaluation of chromosomal radiosensitivity in breast cancer (BC) patients (n = 37) and healthy women (n = 44). METHODS: Chromosomal radiosensitivity was assessed with G0 and G2 assay. For G0 assay lymphocytes were exposed in vitro to 1,5 Gy of X-rays before culture setting. For G2 assay lymphocytes were irradiated with 0,5 Gy of X-rays after 47 h of incubation. RESULTS: Significant differences in mean scores both of G0 and G2 assay between breast cancer patients and controls were observed indicating the increased chromosomal radiosensitivity of lymphocytes of cancer patients. 11% of healthy women and 38% of BC patients were determined to be radiosensitive with G2 assay. CONCLUSION: Obtained results support the concept of association between elevated individual G2 chromosomal radiosensitivity and predisposition to BC.
AIM: Recent studies showed that increased chromosomal damage induced by ionizing radiation is observed among patients with different tumor types. The aim of the study was evaluation of chromosomal radiosensitivity in breast cancer (BC) patients (n = 37) and healthy women (n = 44). METHODS: Chromosomal radiosensitivity was assessed with G0 and G2 assay. For G0 assay lymphocytes were exposed in vitro to 1,5 Gy of X-rays before culture setting. For G2 assay lymphocytes were irradiated with 0,5 Gy of X-rays after 47 h of incubation. RESULTS: Significant differences in mean scores both of G0 and G2 assay between breast cancerpatients and controls were observed indicating the increased chromosomal radiosensitivity of lymphocytes of cancerpatients. 11% of healthy women and 38% of BC patients were determined to be radiosensitive with G2 assay. CONCLUSION: Obtained results support the concept of association between elevated individual G2 chromosomal radiosensitivity and predisposition to BC.
Authors: Leila Dorling; Gillian C Barnett; Kyriaki Michailidou; Charlotte E Coles; Neil G Burnet; John Yarnold; Rebecca M Elliott; Alison M Dunning; Paul D P Pharoah; Catharine M West Journal: Clin Cancer Res Date: 2015-10-28 Impact factor: 12.531
Authors: Juras Kišonas; Jonas Venius; Olga Sevriukova; Mindaugas Grybauskas; Daiva Dabkevičienė; Arvydas Burneckis; Ričardas Rotomskis Journal: Life (Basel) Date: 2021-12-23