Literature DB >> 2300565

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts.

K R Harkins1, R A Jefferson, T A Kavanagh, M W Bevan, D W Galbraith.   

Abstract

We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

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Year:  1990        PMID： 2300565      PMCID： PMC53357          DOI： 10.1073/pnas.87.2.816

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

1.  Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors.

Authors:  J Simpson; M VAN Montagu; L Herrera-Estrella
Journal:  Science       Date:  1986-07-04       Impact factor: 47.728

2.  Identification of upstream regulatory elements involved in the developmental expression of the Arabidopsis thaliana cab1 gene.

Authors:  S B Ha; G An
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

3.  Targeting a foreign protein to chloroplasts using fusions to the transit peptide of a chlorophyll a/b protein.

Authors:  T A Kavanagh; R A Jefferson; M W Bevan
Journal:  Mol Gen Genet       Date:  1988-12

4.  Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

Authors:  F Toneguzzo; A C Hayday; A Keating
Journal:  Mol Cell Biol       Date:  1986-02       Impact factor: 4.272

5.  Factors governing the flow cytometric analysis and sorting of large biological particles.

Authors:  K R Harkins; D W Galbraith
Journal:  Cytometry       Date:  1987-01

6.  Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.

Authors:  B Rotman; B W Papermaster
Journal:  Proc Natl Acad Sci U S A       Date:  1966-01       Impact factor: 11.205

7.  Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector.

Authors:  L Herrera-Estrella; G Van den Broeck; R Maenhaut; M Van Montagu; J Schell; M Timko; A Cashmore
Journal:  Nature       Date:  1984 Jul 12-18       Impact factor: 49.962

8.  Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.

Authors:  A Nordheim; E M Lafer; L J Peck; J C Wang; B D Stollar; A Rich
Journal:  Cell       Date:  1982-12       Impact factor: 41.582

9.  Flow cytometric characterization of the chlorophyll contents and size distributions of plant protoplasts.

Authors:  D W Galbraith; K R Harkins; R A Jefferson
Journal:  Cytometry       Date:  1988-01

10.  Parsley protoplasts retain differential responsiveness to u.v. light and fungal elicitor.

Authors:  J L Dangl; K D Hauffe; S Lipphardt; K Hahlbrock; D Scheel
Journal:  EMBO J       Date:  1987-09       Impact factor: 11.598
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  11 in total

1.  The Role of Plastids in the Expression of Nuclear Genes for Thylakoid Proteins Studied with Chimeric [beta]-Glucuronidase Gene Fusions.

Authors:  C. Bolle; S. Sopory; T. Lubberstedt; R. B. Klosgen; R. G. Herrmann; R. Oelmuller
Journal:  Plant Physiol       Date:  1994-08       Impact factor: 8.340

2.  Biosynthesis, processing and targeting of the G-protein of vesicular stomatitis virus in tobacco protoplasts.

Authors:  D W Galbraith; C A Zeiher; K R Harkins; C L Afonso
Journal:  Planta       Date:  1992-02       Impact factor: 4.116

3.  Z-membranes: artificial organelles for overexpressing recombinant integral membrane proteins.

Authors:  F C Gong; T H Giddings; J B Meehl; L A Staehelin; D W Galbraith
Journal:  Proc Natl Acad Sci U S A       Date:  1996-03-05       Impact factor: 11.205

4.  The promoter from the rice nuclear gene encoding chloroplast aldolase confers mesophyll-specific and light-regulated expression in transgenic tobacco.

Authors:  Y Kagaya; H Nakamura; S Hidaka; S Ejiri; K Tsutsumi
Journal:  Mol Gen Genet       Date:  1995-10-25

5.  Lhcb Transcription Is Coordinated with Cell Size and Chlorophyll Accumulation (Studies on Fluorescence-Activated, Cell-Sorter-Purified Single Cells from Wild-Type and immutans Arabidopsis thaliana).

Authors:  L. Meehan; K. Harkins; J. Chory; S. Rodermel
Journal:  Plant Physiol       Date:  1996-11       Impact factor: 8.340

6.  Potato granule-bound starch synthase promoter-controlled GUS expression: regulation of expression after transient and stable transformation.

Authors:  G van der Steege; M Nieboer; J Swaving; M J Tempelaar
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

Review 7.  Technologies for systems-level analysis of specific cell types in plants.

Authors:  Dongxue Wang; E Shannon Mills; Roger B Deal
Journal:  Plant Sci       Date:  2012-08-30       Impact factor: 4.729

8.  Relationship between protoplast size and critical field strength in protoplast electropulsing and application to reliable DNA uptake in Brassica.

Authors:  D Rouan; M H Montané; G Alibert; J Teissié
Journal:  Plant Cell Rep       Date:  1991-06       Impact factor: 4.570

9.  Global analysis of cell type-specific gene expression.

Authors:  David W Galbraith
Journal:  Comp Funct Genomics       Date:  2003

10.  Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting.

Authors:  Jesper T Grønlund; Alison Eyres; Sanjeev Kumar; Vicky Buchanan-Wollaston; Miriam L Gifford
Journal:  J Vis Exp       Date:  2012-10-04       Impact factor: 1.355
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