BACKGROUND/ PURPOSE: Hypoxia and ischemia appear to play an important role in the pathogenesis of necrotizing enterocolitis (NEC), which may be related to oxygen-derived free radical formation. This study was designed to evaluate the role of oxidative stress and potentially beneficial effects of N-acetylcysteine (NAC) in a neonatal rat model of NEC. METHODS: Thirty Wistar albino rat pups were randomly divided into 3 groups: group 1, control; group 2, NEC and saline; and group 3, NEC and NAC treatment. Necrotizing enterocolitis was induced by hyperosmolar enteral formula feeding and exposure to hypoxia after cold stress at 4°C and oxygen. The pups were killed on the fourth day, and their intestinal tissues were harvested for biochemical and histopathologic analysis. RESULTS: Mucosal injury scores and intestinal malondialdehyde levels in group 2 were found to be significantly higher than that in other groups (P ≤ .05). Intestinal superoxide dismutase activities in group 3 were significantly higher than that in group 2 (P = .018). Intestinal tissue tumor necrosis factor α levels were significantly reduced with NAC treatment in group 3 compared with group 2 (P < .003). CONCLUSIONS: It is likely that oxidative stress and inflammatory mediators contribute to the pathogenesis of NEC and that NAC has a protective effect on intestinal injury through its antiinflammatory and antioxidant properties.
BACKGROUND/ PURPOSE:Hypoxia and ischemia appear to play an important role in the pathogenesis of necrotizing enterocolitis (NEC), which may be related to oxygen-derived free radical formation. This study was designed to evaluate the role of oxidative stress and potentially beneficial effects of N-acetylcysteine (NAC) in a neonatal rat model of NEC. METHODS: Thirty Wistar albino rat pups were randomly divided into 3 groups: group 1, control; group 2, NEC and saline; and group 3, NEC and NAC treatment. Necrotizing enterocolitis was induced by hyperosmolar enteral formula feeding and exposure to hypoxia after cold stress at 4°C and oxygen. The pups were killed on the fourth day, and their intestinal tissues were harvested for biochemical and histopathologic analysis. RESULTS:Mucosal injury scores and intestinal malondialdehyde levels in group 2 were found to be significantly higher than that in other groups (P ≤ .05). Intestinal superoxide dismutase activities in group 3 were significantly higher than that in group 2 (P = .018). Intestinal tissue tumor necrosis factor α levels were significantly reduced with NAC treatment in group 3 compared with group 2 (P < .003). CONCLUSIONS: It is likely that oxidative stress and inflammatory mediators contribute to the pathogenesis of NEC and that NAC has a protective effect on intestinal injury through its antiinflammatory and antioxidant properties.
Authors: Venkatesh Sampath; Daniel Helbling; Heather Menden; David Dimmock; Neil P Mulrooney; Jeffrey C Murray; John M Dagle; Jeffery S Garland Journal: J Pediatr Gastroenterol Nutr Date: 2016-03 Impact factor: 2.839
Authors: Brian P Blackwood; Douglas R Wood; Carrie Yuan; Joseph Nicolas; Isabelle G De Plaen; Kathryn N Farrow; Pauline Chou; Jerrold R Turner; Catherine J Hunter Journal: Am J Pathol Date: 2016-12-08 Impact factor: 4.307
Authors: Soraia Guerra Silvares; Antonio Fernandes Moron; Manuel de Jesus Simões; Álvaro Ulhoa Cintra; Edna Frasson de Souza Montero; Edward Araujo Júnior; José Luiz Martins Journal: Acta Cir Bras Date: 2020-06-05 Impact factor: 1.388