| Literature DB >> 22959267 |
Folkert J van Werven1, Gregor Neuert, Natalie Hendrick, Aurélie Lardenois, Stephen Buratowski, Alexander van Oudenaarden, Michael Primig, Angelika Amon.
Abstract
The cell-fate decision leading to gametogenesis is essential for sexual reproduction. In S. cerevisiae, only diploid MATa/α but not haploid MATa or MATα cells undergo gametogenesis, known as sporulation. We find that transcription of two long noncoding RNAs (lncRNAs) mediates mating-type control of sporulation. In MATa or MATα haploids, expression of IME1, the central inducer of gametogenesis, is inhibited in cis by transcription of the lncRNA IRT1, located in the IME1 promoter. IRT1 transcription recruits the Set2 histone methyltransferase and the Set3 histone deacetylase complex to establish repressive chromatin at the IME1 promoter. Inhibiting expression of IRT1 and an antisense transcript that antagonizes the expression of the meiotic regulator IME4 allows cells expressing the haploid mating type to sporulate with kinetics that are indistinguishable from that of MATa/α diploids. Conversely, expression of the two lncRNAs abolishes sporulation in MATa/α diploids. Thus, transcription of two lncRNAs governs mating-type control of gametogenesis in yeast.Entities:
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Year: 2012 PMID： 22959267 PMCID： PMC3472370 DOI： 10.1016/j.cell.2012.06.049
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582