| Literature DB >> 22941364 |
Michael T Certo1, Kamila S Gwiazda, Ryan Kuhar, Blythe Sather, Gabrielle Curinga, Tyler Mandt, Michelle Brault, Abigail R Lambert, Sarah K Baxter, Kyle Jacoby, Byoung Y Ryu, Hans-Peter Kiem, Agnes Gouble, Frederic Paques, David J Rawlings, Andrew M Scharenberg.
Abstract
Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.Entities:
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Year: 2012 PMID： 22941364 PMCID： PMC3602999 DOI： 10.1038/nmeth.2177
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547