Literature DB >> 19798082

Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays.

Morgan L Maeder1, Stacey Thibodeau-Beganny, Jeffry D Sander, Daniel F Voytas, J Keith Joung.   

Abstract

Engineered zinc-finger nucleases (ZFNs) form the basis of a broadly applicable method for targeted, efficient modification of eukaryotic genomes. In recent work, we described OPEN (oligomerized pool engineering), an 'open-source,' combinatorial selection-based method for engineering zinc-finger arrays that function well as ZFNs. We have also shown in direct comparisons that the OPEN method has a higher success rate than previously described 'modular-assembly' methods for engineering ZFNs. OPEN selections are carried out in Escherichia coli using a bacterial two-hybrid system and do not require specialized equipment. Here we provide a detailed protocol for carrying out OPEN to engineer zinc-finger arrays that have a high probability of functioning as ZFNs. Using OPEN, researchers can generate multiple, customized ZFNs in approximately 8 weeks.

Entities:  

Mesh：

Year:  2009        PMID： 19798082      PMCID： PMC2858690          DOI： 10.1038/nprot.2009.98

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  78 in total

1.  Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins.

Authors:  David J Segal; Roger R Beerli; Pilar Blancafort; Birgit Dreier; Karin Effertz; Adrian Huber; Beate Koksch; Caren V Lund; Laurent Magnenat; David Valente; Carlos F Barbas
Journal:  Biochemistry       Date:  2003-02-25       Impact factor: 3.162

2.  The use of zinc finger peptides to study the role of specific factor binding sites in the chromatin environment.

Authors:  David J Segal
Journal:  Methods       Date:  2002-01       Impact factor: 3.608

3.  Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases.

Authors:  Marina Bibikova; Mary Golic; Kent G Golic; Dana Carroll
Journal:  Genetics       Date:  2002-07       Impact factor: 4.562

4.  Controlling gene expression in plants using synthetic zinc finger transcription factors.

Authors:  Justin T Stege; Xuen Guan; Thao Ho; Roger N Beachy; Carlos F Barbas
Journal:  Plant J       Date:  2002-12       Impact factor: 6.417

5.  Regulation of transgene expression in plants with polydactyl zinc finger transcription factors.

Authors:  M Isabel Ordiz; Carlos F Barbas; Roger N Beachy
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-23       Impact factor: 11.205

6.  Induction of angiogenesis in a mouse model using engineered transcription factors.

Authors:  Edward J Rebar; Yan Huang; Reed Hickey; Anjali K Nath; David Meoli; Sameer Nath; Bingliang Chen; Lei Xu; Yuxin Liang; Andrew C Jamieson; Lei Zhang; S Kaye Spratt; Casey C Case; Alan Wolffe; Frank J Giordano
Journal:  Nat Med       Date:  2002-11-04       Impact factor: 53.440

7.  Activation of vascular endothelial growth factor A transcription in tumorigenic glioblastoma cell lines by an enhancer with cell type-specific DNase I accessibility.

Authors:  Yuxin Liang; Xiao-Yong Li; Edward J Rebar; Peixiang Li; Yuanyue Zhou; Bingliang Chen; Alan P Wolffe; Casey C Case
Journal:  J Biol Chem       Date:  2002-03-23       Impact factor: 5.157

8.  Human zinc fingers as building blocks in the construction of artificial transcription factors.

Authors:  Kwang-Hee Bae; Young Do Kwon; Hyun-Chul Shin; Moon-Sun Hwang; Eun-Hyun Ryu; Kyung-Soon Park; Hyo-Young Yang; Dong-Ki Lee; Yangsoon Lee; Jinwoo Park; Heung Sun Kwon; Hyun-Won Kim; Byung-Il Yeh; Hyean-Woo Lee; Soon Hyung Sohn; Joonho Yoon; Wongi Seol; Jin-Soo Kim
Journal:  Nat Biotechnol       Date:  2003-02-18       Impact factor: 54.908

9.  Scanning the human genome with combinatorial transcription factor libraries.

Authors:  Pilar Blancafort; Laurent Magnenat; Carlos F Barbas
Journal:  Nat Biotechnol       Date:  2003-02-18       Impact factor: 54.908

10.  Heritable endogenous gene regulation in plants with designed polydactyl zinc finger transcription factors.

Authors:  Xuen Guan; Justin Stege; Myoung Kim; Zina Dahmani; Nancy Fan; Peter Heifetz; Carlos F Barbas; Steven P Briggs
Journal:  Proc Natl Acad Sci U S A       Date:  2002-09-23       Impact factor: 11.205
View more
  82 in total

Review 1.  Zinc-finger nucleases for somatic gene therapy: the next frontier.

Authors:  Shamim H Rahman; Morgan L Maeder; J Keith Joung; Toni Cathomen
Journal:  Hum Gene Ther       Date:  2011-07-22       Impact factor: 5.695

2.  Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo.

Authors:  Alison A Staton; Antonio J Giraldez
Journal:  Nat Protoc       Date:  2011-12-01       Impact factor: 13.491

3.  Retargeting sleeping beauty transposon insertions by engineered zinc finger DNA-binding domains.

Authors:  Katrin Voigt; Andreas Gogol-Döring; Csaba Miskey; Wei Chen; Toni Cathomen; Zsuzsanna Izsvák; Zoltán Ivics
Journal:  Mol Ther       Date:  2012-07-10       Impact factor: 11.454

4.  High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases.

Authors:  Feng Zhang; Morgan L Maeder; Erica Unger-Wallace; Justin P Hoshaw; Deepak Reyon; Michelle Christian; Xiaohong Li; Christopher J Pierick; Drena Dobbs; Thomas Peterson; J Keith Joung; Daniel F Voytas
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-27       Impact factor: 11.205

5.  Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells.

Authors:  Jia Liu; Thomas Gaj; Yifeng Yang; Nan Wang; Sailan Shui; Sojung Kim; Chidananda Nagamangala Kanchiswamy; Jin-Soo Kim; Carlos F Barbas
Journal:  Nat Protoc       Date:  2015-10-22       Impact factor: 13.491

Review 6.  Genetic therapies for cystic fibrosis lung disease.

Authors:  Patrick L Sinn; Reshma M Anthony; Paul B McCray
Journal:  Hum Mol Genet       Date:  2011-03-21       Impact factor: 6.150

Review 7.  Genome editing: a robust technology for human stem cells.

Authors:  Arun Pandian Chandrasekaran; Minjung Song; Suresh Ramakrishna
Journal:  Cell Mol Life Sci       Date:  2017-04-12       Impact factor: 9.261

Review 8.  Progress and prospects of engineered sequence-specific DNA modulating technologies for the management of liver diseases.

Authors:  Samantha A Nicholson; Buhle Moyo; Patrick B Arbuthnot
Journal:  World J Hepatol       Date:  2015-04-28

9.  Targeted mutagenesis in zebrafish using customized zinc-finger nucleases.

Authors:  Jonathan E Foley; Morgan L Maeder; Joseph Pearlberg; J Keith Joung; Randall T Peterson; Jing-Ruey J Yeh
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

Review 10.  Zebrafish as a disease model for studying human hepatocellular carcinoma.

Authors:  Jeng-Wei Lu; Yi-Jung Ho; Yi-Ju Yang; Heng-An Liao; Shih-Ci Ciou; Liang-In Lin; Da-Liang Ou
Journal:  World J Gastroenterol       Date:  2015-11-14       Impact factor: 5.742
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.