Literature DB >> 22923414

Iterative combinatorial mutagenesis as an effective strategy for generation of deacetoxycephalosporin C synthase with improved activity toward penicillin G.

Junjie Ji1, Keqiang Fan, Xiuyun Tian, Xia Zhang, Yuxiu Zhang, Keqian Yang.   

Abstract

An iterative combinatorial mutagenesis (ICM) strategy was used to engineer deacetoxycephalosporin C synthase of Streptomyces clavuligerus (scDAOCS) for improved activity toward penicillin G. Seven mutational sites were repeatedly combined onto a starter mutant (C155Y Y184H V275I C281Y) of scDAOCS. Eleven improved combinatorial mutants were identified from 24 mutants in four rounds of ICM.

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Year:  2012        PMID： 22923414      PMCID： PMC3485735          DOI： 10.1128/AEM.02122-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G.

Authors:  Chia-Li Wei; Yunn-Bor Yang; Wen-Ching Wang; Wen-Chi Liu; Jyh-Shing Hsu; Ying-Chieh Tsai
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

2.  Environmentally safe production of 7-aminodeacetoxycephalosporanic acid (7-ADCA) using recombinant strains of Acremonium chrysogenum.

Authors:  J Velasco; J Luis Adrio; M Angel Moreno; B Díez; G Soler; J L Barredo
Journal:  Nat Biotechnol       Date:  2000-08       Impact factor: 54.908

3.  A complete library of amino acid alterations at N304 in Streptomyces clavuligerus deacetoxycephalosporin C synthase elucidates the basis for enhanced penicillin analogue conversion.

Authors:  Hong Soon Chin; Kian Sim Goo; T S Sim
Journal:  Appl Environ Microbiol       Date:  2004-01       Impact factor: 4.792

4.  Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods.

Authors:  Manfred T Reetz; Shreenath Prasad; José D Carballeira; Yosephine Gumulya; Marco Bocola
Journal:  J Am Chem Soc       Date:  2010-07-07       Impact factor: 15.419

5.  Saturation mutagenesis of Acremonium chrysogenum deacetoxy/deacetylcephalosporin C synthase R308 site confirms its role in controlling substrate specificity.

Authors:  Xiao-Bin Wu; Xiu-Yun Tian; Jun-Jie Ji; Wei-Bin Wu; Ke-Qiang Fan; Ke-Qian Yang
Journal:  Biotechnol Lett       Date:  2010-12-29       Impact factor: 2.461

6.  Learning from directed evolution: Further lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity.

Authors:  Manfred T Reetz; Michael Puls; José Daniel Carballeira; Andreas Vogel; Karl-Erich Jaeger; Thorsten Eggert; Walter Thiel; Marco Bocola; Nikolaj Otte
Journal:  Chembiochem       Date:  2007-01-02       Impact factor: 3.164

7.  Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes.

Authors:  Manfred T Reetz; José Daniel Carballeira
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

8.  New strategy of site-directed mutagenesis identifies new sites to improve Streptomyces clavuligerus deacetoxycephalosporin C synthase activity toward penicillin G.

Authors:  Junjie Ji; Xiuyun Tian; Keqiang Fan; Keqian Yang
Journal:  Appl Microbiol Biotechnol       Date:  2011-09-10       Impact factor: 4.813

Review 9.  Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.

Authors:  Kian-Sim Goo; Chun-Song Chua; Tiow-Suan Sim
Journal:  J Ind Microbiol Biotechnol       Date:  2009-03-07       Impact factor: 3.346

10.  The structural basis of cephalosporin formation in a mononuclear ferrous enzyme.

Authors:  Karin Valegård; Anke C Terwisscha van Scheltinga; Alain Dubus; Graziella Ranghino; Linda M Oster; Janos Hajdu; Inger Andersson
Journal:  Nat Struct Mol Biol       Date:  2003-12-29       Impact factor: 15.369
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  5 in total

Review 1.  Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins.

Authors:  Keqiang Fan; Baixue Lin; Yong Tao; Keqian Yang
Journal:  J Ind Microbiol Biotechnol       Date:  2016-11-08       Impact factor: 3.346

2.  Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G.

Authors:  Baixue Lin; Keqiang Fan; Jian Zhao; Junjie Ji; Linjun Wu; Keqian Yang; Yong Tao
Journal:  Proc Natl Acad Sci U S A       Date:  2015-07-27       Impact factor: 11.205

Review 3.  Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in β-lactam biosynthesis.

Authors:  Patrick Rabe; Jos J A G Kamps; Christopher J Schofield; Christopher T Lohans
Journal:  Nat Prod Rep       Date:  2018-08-15       Impact factor: 13.423

4.  Deacetoxycephalosporin C synthase (expandase): Research progress and application potential.

Authors:  Xiaofan Niu; Jian Zhang; Xianli Xue; Depei Wang; Lin Wang; Qiang Gao
Journal:  Synth Syst Biotechnol       Date:  2021-11-23

Review 5.  Making Enzymes Suitable for Organic Chemistry by Rational Protein Design.

Authors:  Manfred Reetz
Journal:  Chembiochem       Date:  2022-04-27       Impact factor: 3.461
  5 in total

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