Literature DB >> 14711695

A complete library of amino acid alterations at N304 in Streptomyces clavuligerus deacetoxycephalosporin C synthase elucidates the basis for enhanced penicillin analogue conversion.

Hong Soon Chin1, Kian Sim Goo, T S Sim.   

Abstract

N304 of Streptomyces clavuligerus deacetoxycephalosporin C synthase was mutagenized to alter its catalytic ability. Given that N304A, N304K, N304L, and N304R mutant enzymes exhibited significant improvements in penicillin analogue conversions, we advocate that replacement of N304 with residues with aliphatic or basic side chains is preferable for engineering of a hypercatalytic enzyme.

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Year:  2004        PMID： 14711695      PMCID： PMC321265          DOI： 10.1128/AEM.70.1.607-609.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

Review 1.  The hunchback and its neighbours: proline as an environmental modulator.

Authors:  H Reiersen; A R Rees
Journal:  Trends Biochem Sci       Date:  2001-11       Impact factor: 13.807

2.  Mutation of N304 to leucine in Streptomyces clavuligerus deacetoxycephalosporin C synthase creates an enzyme with increased penicillin analogue conversion.

Authors:  H S Chin; J Sim; T S Sim
Journal:  Biochem Biophys Res Commun       Date:  2001-09-21       Impact factor: 3.575

Review 3.  The iron(II) and 2-oxoacid-dependent dioxygenases and their role in metabolism.

Authors:  A G Prescott; M D Lloyd
Journal:  Nat Prod Rep       Date:  2000-08       Impact factor: 13.423

4.  Engineering Streptomyces clavuligerus deacetoxycephalosporin C synthase for optimal ring expansion activity toward penicillin G.

Authors:  Chia-Li Wei; Yunn-Bor Yang; Wen-Ching Wang; Wen-Chi Liu; Jyh-Shing Hsu; Ying-Chieh Tsai
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

5.  Deacetoxycephalosporin C synthase isozymes exhibit diverse catalytic activity and substrate specificity.

Authors:  Hong Soon Chin; Janet Sim; Keng Ing Seah; Tiow Suan Sim
Journal:  FEMS Microbiol Lett       Date:  2003-01-28       Impact factor: 2.742

6.  Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS).

Authors:  H J Lee; M D Lloyd; K Harlos; I J Clifton; J E Baldwin; C J Schofield
Journal:  J Mol Biol       Date:  2001-05-18       Impact factor: 5.469

7.  Environmentally safe production of 7-aminodeacetoxycephalosporanic acid (7-ADCA) using recombinant strains of Acremonium chrysogenum.

Authors:  J Velasco; J Luis Adrio; M Angel Moreno; B Díez; G Soler; J L Barredo
Journal:  Nat Biotechnol       Date:  2000-08       Impact factor: 54.908

8.  Studies on the active site of deacetoxycephalosporin C synthase.

Authors:  M D Lloyd; H J Lee; K Harlos; Z H Zhang; J E Baldwin; C J Schofield; J M Charnock; C D Garner; T Hara; A C Terwisscha van Scheltinga; K Valegård; J A Viklund; J Hajdu; I Andersson; A Danielsson; R Bhikhabhai
Journal:  J Mol Biol       Date:  1999-04-16       Impact factor: 5.469

Review 9.  The cephalosporins.

Authors:  W F Marshall; J E Blair
Journal:  Mayo Clin Proc       Date:  1999-02       Impact factor: 7.616

Review 10.  The enzymes involved in biosynthesis of penicillin and cephalosporin; their structure and function.

Authors:  R D Cooper
Journal:  Bioorg Med Chem       Date:  1993-07       Impact factor: 3.641
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  7 in total

1.  Relevant double mutations in bioengineered Streptomyces clavuligerus deacetoxycephalosporin C synthase result in higher binding specificities which improve penicillin bioconversion.

Authors:  Kian Sim Goo; Chun Song Chua; Tiow-Suan Sim
Journal:  Appl Environ Microbiol       Date:  2007-12-14       Impact factor: 4.792

2.  Iterative combinatorial mutagenesis as an effective strategy for generation of deacetoxycephalosporin C synthase with improved activity toward penicillin G.

Authors:  Junjie Ji; Keqiang Fan; Xiuyun Tian; Xia Zhang; Yuxiu Zhang; Keqian Yang
Journal:  Appl Environ Microbiol       Date:  2012-08-24       Impact factor: 4.792

3.  Characterization of Plasmodium falciparum co-chaperone p23: its intrinsic chaperone activity and interaction with Hsp90.

Authors:  Chun-Song Chua; Huiyu Low; Kian-Sim Goo; T S Sim
Journal:  Cell Mol Life Sci       Date:  2010-02-06       Impact factor: 9.261

Review 4.  Directed evolution and rational approaches to improving Streptomyces clavuligerus deacetoxycephalosporin C synthase for cephalosporin production.

Authors:  Kian-Sim Goo; Chun-Song Chua; Tiow-Suan Sim
Journal:  J Ind Microbiol Biotechnol       Date:  2009-03-07       Impact factor: 3.346

Review 5.  Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins.

Authors:  Keqiang Fan; Baixue Lin; Yong Tao; Keqian Yang
Journal:  J Ind Microbiol Biotechnol       Date:  2016-11-08       Impact factor: 3.346

Review 6.  Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in β-lactam biosynthesis.

Authors:  Patrick Rabe; Jos J A G Kamps; Christopher J Schofield; Christopher T Lohans
Journal:  Nat Prod Rep       Date:  2018-08-15       Impact factor: 13.423

7.  Deacetoxycephalosporin C synthase (expandase): Research progress and application potential.

Authors:  Xiaofan Niu; Jian Zhang; Xianli Xue; Depei Wang; Lin Wang; Qiang Gao
Journal:  Synth Syst Biotechnol       Date:  2021-11-23
  7 in total

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