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Literature DB >> 17400785

High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni.

Erin P Price¹, Helen Smith, Flavia Huygens, Philip M Giffard.

Abstract

A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species.

Entities: Species

Mesh：

Substances：
DNA, Bacterial

Year: 2007 PMID： 17400785 PMCID： PMC1907115 DOI： 10.1128/AEM.02702-06

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

20 in total

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4. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences.

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Journal: Nature Date: 2000-02-10 Impact factor: 49.962

5. Identification and interrogation of highly informative single nucleotide polymorphism sets defined by bacterial multilocus sequence typing databases.

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6. mecA Locus diversity in methicillin-resistant Staphylococcus aureus isolates in Brisbane, Australia, and the development of a novel diagnostic procedure for the Western Samoan phage pattern clone.

Authors: Flavia Huygens; Alex J Stephens; Graeme R Nimmo; Philip M Giffard
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7. Comparative genotyping of Campylobacter jejuni by amplified fragment length polymorphism, multilocus sequence typing, and short repeat sequencing: strain diversity, host range, and recombination.

Authors: Leo M Schouls; Sanne Reulen; Birgitta Duim; Jaap A Wagenaar; Rob J L Willems; Kate E Dingle; Frances M Colles; Jan D A Van Embden
Journal: J Clin Microbiol Date: 2003-01 Impact factor: 5.948

8. High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS codon 12 and 13 mutations in non-small cell lung cancer.

Authors: Michael Krypuy; Genni M Newnham; David M Thomas; Matthew Conron; Alexander Dobrovic
Journal: BMC Cancer Date: 2006-12-21 Impact factor: 4.430

9. Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species.

Authors: Derrick E Fouts; Emmanuel F Mongodin; Robert E Mandrell; William G Miller; David A Rasko; Jacques Ravel; Lauren M Brinkac; Robert T DeBoy; Craig T Parker; Sean C Daugherty; Robert J Dodson; A Scott Durkin; Ramana Madupu; Steven A Sullivan; Jyoti U Shetty; Mobolanle A Ayodeji; Alla Shvartsbeyn; Michael C Schatz; Jonathan H Badger; Claire M Fraser; Karen E Nelson
Journal: PLoS Biol Date: 2005-01-04 Impact factor: 8.029

10. mlstdbNet - distributed multi-locus sequence typing (MLST) databases.

Authors: Keith A Jolley; Man-Suen Chan; Martin C J Maiden
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37 in total

1. Rapid detection of isoniazid, rifampin, and ofloxacin resistance in Mycobacterium tuberculosis clinical isolates using high-resolution melting analysis.

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Review 4. Microbiological applications of high-resolution melting analysis.

Authors: Steven Y C Tong; Philip M Giffard
Journal: J Clin Microbiol Date: 2012-08-08 Impact factor: 5.948

5. Molecular identification and characterization of clustered regularly interspaced short palindromic repeats (CRISPRs) in a urease-positive thermophilic Campylobacter sp. (UPTC).

Authors: E Tasaki; J Hirayama; A Tazumi; K Hayashi; Y Hara; H Ueno; J E Moore; B C Millar; M Matsuda
Journal: World J Microbiol Biotechnol Date: 2011-08-28 Impact factor: 3.312