Literature DB >> 22864909

qRT-PCR: a method and its difficulties.

Franziska Bollmann, Ingrid Casper, Jenny Henke, Andrea Pautz.   

Abstract

Mesh：

Substances：

Year:  2012        PMID： 22864909     DOI： 10.1007/s00210-012-0786-3

Source DB:  PubMed          Journal:  Naunyn Schmiedebergs Arch Pharmacol        ISSN： 0028-1298            Impact factor:   3.000


× No keyword cloud information.
  15 in total

1.  Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.

Authors:  Michael W Pfaffl; Ales Tichopad; Christian Prgomet; Tanja P Neuvians
Journal:  Biotechnol Lett       Date:  2004-03       Impact factor: 2.461

2.  The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

Authors:  Stephen A Bustin; Vladimir Benes; Jeremy A Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory L Shipley; Jo Vandesompele; Carl T Wittwer
Journal:  Clin Chem       Date:  2009-02-26       Impact factor: 8.327

3.  Eleven golden rules of quantitative RT-PCR.

Authors:  Michael K Udvardi; Tomasz Czechowski; Wolf-Rüdiger Scheible
Journal:  Plant Cell       Date:  2008-07-29       Impact factor: 11.277

Review 4.  Normalization of qRT-PCR data: the necessity of adopting a systematic, experimental conditions-specific, validation of references.

Authors:  Stéphanie Guénin; Mélanie Mauriat; Jérôme Pelloux; Olivier Van Wuytswinkel; Catherine Bellini; Laurent Gutierrez
Journal:  J Exp Bot       Date:  2009       Impact factor: 6.992

5.  Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

Authors:  Frank Vandenbussche; Elise Vandemeulebroucke; Kris De Clercq
Journal:  Methods Mol Biol       Date:  2010

6.  Tissue-specific selection of stable reference genes for real-time PCR normalization in an obese rat model.

Authors:  Manuela Cabiati; Serena Raucci; Chiara Caselli; Maria Angela Guzzardi; Andrea D'Amico; Tommaso Prescimone; Daniela Giannessi; Silvia Del Ry
Journal:  J Mol Endocrinol       Date:  2012-05-08       Impact factor: 5.098

7.  A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues.

Authors:  Elena A Takano; Thomas Mikeska; Alexander Dobrovic; David J Byrne; Stephen B Fox
Journal:  BMC Biotechnol       Date:  2010-12-17       Impact factor: 2.563

8.  Impact of reference gene selection for target gene normalization on experimental outcome using real-time qRT-PCR in adipocytes.

Authors:  Bradley S Ferguson; Heesun Nam; Robin G Hopkins; Ron F Morrison
Journal:  PLoS One       Date:  2010-12-13       Impact factor: 3.240

9.  Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Authors:  Jo Vandesompele; Katleen De Preter; Filip Pattyn; Bruce Poppe; Nadine Van Roy; Anne De Paepe; Frank Speleman
Journal:  Genome Biol       Date:  2002-06-18       Impact factor: 13.583

10.  Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries.

Authors:  Samuel T Workenhe; Molly J T Kibenge; Tokinori Iwamoto; Frederick S B Kibenge
Journal:  J Virol Methods       Date:  2008-09-26       Impact factor: 2.014
View more
  7 in total

1.  Regulation of GAPDH expression by treatment with the β-adrenoceptor agonist isoprenaline--is GADPH a suitable loading control in immunoblot experiments?

Authors:  Martina B Michel-Reher; Martin C Michel
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2015-08-18       Impact factor: 3.000

2.  Easy-to-use strategy for reference gene selection in quantitative real-time PCR experiments.

Authors:  Stefanie Klenke; Kristina Renckhoff; Andrea Engler; Jürgen Peters; Ulrich H Frey
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2016-09-20       Impact factor: 3.000

3.  Identification of suitable internal control genes for transcriptional studies in Eleusine coracana under different abiotic stress conditions.

Authors:  Pradeep K Jatav; Ankita Sharma; Dinesh K Dahiya; Arif Khan; Atika Agarwal; S L Kothari; Sumita Kachhwaha
Journal:  Physiol Mol Biol Plants       Date:  2018-07-04

4.  Flow cytometric analysis with a fluorescently labeled formyl peptide receptor ligand as a new method to study the pharmacological profile of the histamine H2 receptor.

Authors:  Kristin Werner; Solveig Kälble; Sabine Wolter; Erich H Schneider; Armin Buschauer; Detlef Neumann; Roland Seifert
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2015-05-30       Impact factor: 3.000

5.  Muscarinic receptor subtype mRNA expression in the human prostate: association with age, pathological diagnosis, prostate size, or potentially interfering medications?

Authors:  Lambertus P W Witte; Christine A Teitsma; Jean J M C H de la Rosette; Martin C Michel
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2013-11-06       Impact factor: 3.000

6.  Identification of novel and robust internal control genes from Volvariella volvacea that are suitable for RT-qPCR in filamentous fungi.

Authors:  Yongxin Tao; Arend Frans van Peer; Qianhui Huang; Yanping Shao; Lei Zhang; Bin Xie; Yuji Jiang; Jian Zhu; Baogui Xie
Journal:  Sci Rep       Date:  2016-07-12       Impact factor: 4.379

7.  Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions.

Authors:  Jiang Qian; Yingnv Gao; Ying Wáng; Yingying Wu; Ying Wāng; Yucheng Zhao; Hongyu Chen; Dapeng Bao; Jiyang Xu; Xiaohong Bian
Journal:  Biomed Res Int       Date:  2018-07-09       Impact factor: 3.411
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.